Kumagai D, Yamate J, Tajima T, Tsukamoto Y, Yasui H, Kuwamura M, Kotani T, Sakuma S
Veterinary Teaching Hospital, College of Agriculture, Osaka Prefecture University, Gakuencho 1-1, Sakai, Osaka, 599-8531, Japan.
J Comp Pathol. 2000 Aug-Oct;123(2-3):77-87. doi: 10.1053/jcpa.2000.0389.
To pursue the histogenesis of malignant fibrous histiocytoma (MFH), of which the cell of origin is still debated, a monoclonal antibody (A3) was produced against a rat MFH-derived cloned cell line (MT-8). Antigen recognized by A3 was around 80 kDa in molecular weight and was seen on the cytoplasmic membrane of MT-8 cells by immunoelectron microscopy. A3 reacted specifically with MT-8 cells, with another rat MFH-derived cell line (MT-9) and with their induced tumours in syngeneic rats, but not with other rat tumours such as fibrosarcoma, histiocytic sarcoma, malignant meningioma, uterine leiomyosarcoma, endometrial stromal sarcoma, mononuclear cell leukaemia and malignant schwannoma. These findings indicate that A3 has a high specificity for rat MFH cells. In fetuses on gestation days 15, 18 and 20 and in postnatal rats aged 1, 4 and 8 days, A3 reacted with primitive mesenchymal cells in visceral organs and around arteries and bronchi, as well as in the lamina propria of intestinal mucosa, renal interstitium, meninges and perineurium. There were no A3-positive connective tissue cells in organs or other sites in adult rats more than 10 weeks old. It is therefore likely that MFH cells share antigens with primitive mesenchymal cells, which may be multipotent for mesenchymal differentiation. The present study suggests that MFH consists of a population of primitive, undifferentiated mesenchymal cells. A3 also immunolabelled endothelial cells of arteries, venules and pulmonary capillaries in fetal, postnatal and adult rats; vascular endothelial cells in chemically induced hepatic and renal lesions also reacted strongly with A3. However, the significance of endothelial immunoreactivity with A3 remains to be elucidated.
为了探究恶性纤维组织细胞瘤（MFH）的组织发生，其起源细胞仍存在争议，我们制备了一种针对大鼠MFH来源的克隆细胞系（MT - 8）的单克隆抗体（A3）。A3识别的抗原分子量约为80 kDa，免疫电子显微镜观察显示其位于MT - 8细胞的细胞质膜上。A3与MT - 8细胞、另一种大鼠MFH来源的细胞系（MT - 9）及其在同基因大鼠中诱导产生的肿瘤特异性反应，但不与其他大鼠肿瘤反应，如纤维肉瘤、组织细胞肉瘤、恶性脑膜瘤、子宫平滑肌肉瘤、子宫内膜间质肉瘤、单核细胞白血病和恶性神经鞘瘤。这些发现表明A3对大鼠MFH细胞具有高度特异性。在妊娠第15、18和20天的胎儿以及出生后1、4和8天的大鼠中，A3与内脏器官、动脉和支气管周围以及肠黏膜固有层、肾间质、脑膜和神经束膜中的原始间充质细胞反应。在10周龄以上的成年大鼠的器官或其他部位没有A3阳性的结缔组织细胞。因此，MFH细胞可能与原始间充质细胞共享抗原，原始间充质细胞可能对间充质分化具有多能性。本研究表明MFH由一群原始的、未分化的间充质细胞组成。A3还对胎儿、出生后和成年大鼠的动脉、小静脉和肺毛细血管的内皮细胞进行免疫标记；化学诱导的肝和肾损伤中的血管内皮细胞也与A3强烈反应。然而，A3与内皮细胞免疫反应的意义仍有待阐明。
