Mariotti F, Cuteri V, Takai S, Renzoni G, Pascucci L, Vitellozzi G
Dipartimento di Scienze Veterinarie, Università di Camerino, Italia.
J Comp Pathol. 2000 Aug-Oct;123(2-3):186-9. doi: 10.1053/jcpa.2000.0392.
Two horses with Rhodococcus equi infection were examined post mortem by an immunohistochemical method (peroxidase-antiperoxidase; PAP) with a monoclonal antibody (Mab 10G5) to the 15-17 kDa antigen of R. equi. One of the horses was also examined bacteriologically, R. equi being isolated in culture. Immunolabelling with this Mab was marked and widespread. On the other hand, the immunohistochemical reactivity of infected macrophages with a polyclonal antibody specific for lysozyme was slight. Thus, Mab 10G5 would appear to be a useful diagnostic reagent in R. equi infection, with or without cultural confirmation.
对两匹感染马红球菌的马进行了死后检查，采用免疫组织化学方法（过氧化物酶-抗过氧化物酶；PAP），使用针对马红球菌15-17 kDa抗原的单克隆抗体（Mab 10G5）。其中一匹马还进行了细菌学检查，在培养物中分离出了马红球菌。用这种单克隆抗体进行的免疫标记明显且广泛。另一方面，感染巨噬细胞与溶菌酶特异性多克隆抗体的免疫组织化学反应轻微。因此，无论有无培养确认，Mab 10G5似乎都是马红球菌感染的一种有用诊断试剂。
