FGF-20是一种新型神经营养因子，在大鼠脑黑质致密部中优先表达。
FGF-20, a novel neurotrophic factor, preferentially expressed in the substantia nigra pars compacta of rat brain.
作者信息
Ohmachi S, Watanabe Y, Mikami T, Kusu N, Ibi T, Akaike A, Itoh N
机构信息
Department of Genetic Biochemistry, Kyoto University Graduate School of Pharmaceutical Sciences, Sakyo, Kyoto, 606-8501, Japan.
出版信息
Biochem Biophys Res Commun. 2000 Oct 22;277(2):355-60. doi: 10.1006/bbrc.2000.3675.
We have isolated cDNA encoding a novel FGF (212 amino acids) from rat brain. Because this is the 20th documented member of the FGF family, we tentatively term it FGF-20. Among FGF family members, FGF-20 is most similar to FGF-9 and FGF-16 (70 and 62% amino acid identity, respectively). Human FGF-20 gene was found in the human genomic sequence mapped to the 8p21.3-p22 region. Human FGF-20 is highly identical to rat FGF-20 (95% amino acid identity). FGF-20 mRNA was preferentially expressed in rat brain among the adult major tissues examined. The localization of FGF-20 mRNA in rat brain was also examined by in situ hybridization. FGF-20 mRNA was preferentially expressed in the substantia nigra pars compacta. To examine the biological activity of FGF-20, recombinant rat FGF-20 was produced by insect cells infected with recombinant baculovirus containing rat FGF-20 cDNA. Recombinant rat FGF-20 enhanced the survival of midbrain dopaminergic neurons. The present results indicate that FGF-20 is a novel neurotrophic factor preferentially expressed in the substantia nigra pars compacta of rat brain.
我们从大鼠脑中分离出了编码一种新型成纤维细胞生长因子（212个氨基酸）的互补DNA。由于这是成纤维细胞生长因子家族中第20个被记录的成员，我们暂时将其命名为FGF - 20。在成纤维细胞生长因子家族成员中，FGF - 20与FGF - 9和FGF - 16最为相似（氨基酸同一性分别为70%和62%）。在定位到8p21.3 - p22区域的人类基因组序列中发现了人类FGF - 20基因。人类FGF - 20与大鼠FGF - 最相似（氨基酸同一性为95%）。在检测的成年大鼠主要组织中，FGF - 20信使核糖核酸在脑中优先表达。我们还通过原位杂交检测了FGF - 20信使核糖核酸在大鼠脑中的定位。FGF - 20信使核糖核酸在黑质致密部优先表达。为了检测FGF - 20的生物活性，用含有大鼠FGF - 20互补DNA的重组杆状病毒感染昆虫细胞，制备重组大鼠FGF - 20。重组大鼠FGF - 20提高了中脑多巴胺能神经元的存活率。目前的结果表明，FGF - 20是一种新型神经营养因子，在大鼠脑黑质致密部优先表达。
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