Hoshikawa M, Ohbayashi N, Yonamine A, Konishi M, Ozaki K, Fukui S, Itoh N
Department of Genetic Biochemistry, Kyoto University Graduate School of Pharmaceutical Sciences, Japan.
Biochem Biophys Res Commun. 1998 Mar 6;244(1):187-91. doi: 10.1006/bbrc.1998.8239.
We isolated the cDNA encoding a novel member (216 amino acids) of the fibroblast growth factor (FGF) family from rat embryos. As this protein is the 17th documented member of the FGF family, we tentatively termed it FGF-17. We have also determined the structures of mouse and human FGF-17 with high amino acid identity (100 and 98.6%) to rat FGF-17, respectively. Among FGF family members, FGF-17 is most similar (53.7% amino acid identity) to FGF-8. FGF-17 has a typical signal sequence at its amino terminus. As expected, recombinant rat FGF-17 was efficiently secreted by High Five insect cells infected with recombinant baculovirus containing the cDNA indicating that FGF-17 is a secreted protein. FGF-17 mRNA of approximately 2.1 kb was detected in rat embryos at E14.5, but not at E10.5 and E19.5 by Northern analysis. The mRNA was found to be preferentially expressed in the neuroepithelia of the isthmus and septum of the rat embryonic brain at E14.5 by in situ hybridization. The present results indicate that FGF-17 might be a novel secreted signaling molecule in the induction and patterning of the embryonic brain.
我们从大鼠胚胎中分离出了编码成纤维细胞生长因子（FGF）家族一个新成员（216个氨基酸）的cDNA。由于该蛋白是FGF家族中第17个被记录的成员，我们暂时将其命名为FGF - 17。我们还分别确定了与大鼠FGF - 17具有高氨基酸同一性（分别为100%和98.6%）的小鼠和人类FGF - 17的结构。在FGF家族成员中，FGF - 17与FGF - 8最为相似（氨基酸同一性为53.7%）。FGF - 17在其氨基末端有一个典型的信号序列。正如预期的那样，感染了含有该cDNA的重组杆状病毒的High Five昆虫细胞有效地分泌了重组大鼠FGF - 17，这表明FGF - 17是一种分泌蛋白。通过Northern分析在E14.5的大鼠胚胎中检测到了约2.1 kb的FGF - 17 mRNA，但在E10.5和E19.5时未检测到。通过原位杂交发现，在E14.5时，该mRNA在大鼠胚胎脑峡部和中隔的神经上皮中优先表达。目前的结果表明，FGF - 17可能是胚胎脑诱导和模式形成中的一种新型分泌信号分子。
