Frye C A, Manjarrez J, Camacho-Arroyo I
Department of Psychology, Biological Sciences & The Neurobiology Research Center, The University at Albany-SUNY, 1400 Washington Avenue, Albany, NY 12222, USA.
Brain Res. 2000 Oct 20;881(1):98-102. doi: 10.1016/s0006-8993(00)02897-3.
Whether progesterone (P(4)) and its metabolite, 5alpha-pregnan-3alpha-ol-20-one (3alpha,5alpha-THP) have anti-seizure effects through actions in the pontine reticular formation (PRF) was investigated. Concentrations of P(4) and 3alpha, 5alpha-THP in the PRF were greater in proestrous and hormone-primed rats, that are typically more resistant to seizure-induction, than diestrous and males rats. Ovx, Long-Evans rats with unilateral microinjections into the PRF of 3alpha,5alpha-THP (5 microg/0.2 microl), but not P(4) (11 microg/0.2 microl) or vehicle (beta-cyclodextrin), had a greater latency and lower incidence of tonic-clonic seizures induced by pentylenetetrazol (PTZ; 70 mg/kg, IP) administration. Infusions that missed the PRF were not effective. These data suggest 3alpha,5alpha-THP has anti-seizure effects in part through actions in the PRF.
研究了孕酮（P(4)）及其代谢产物5α-孕烷-3α-醇-20-酮（3α,5α-THP）是否通过在脑桥网状结构（PRF）中的作用发挥抗癫痫作用。与动情后期和雄性大鼠相比，动情前期和激素预处理的大鼠（通常对癫痫诱导更具抵抗力）PRF中P(4)和3α,5α-THP的浓度更高。对去卵巢的Long-Evans大鼠单侧脑桥网状结构微量注射3α,5α-THP（5微克/0.2微升），而非P(4)（11微克/0.2微升）或溶剂（β-环糊精），戊四氮（PTZ；70毫克/千克，腹腔注射）诱导的强直阵挛性癫痫发作的潜伏期更长，发生率更低。未注射到PRF的灌注无效。这些数据表明3α,5α-THP部分通过在PRF中的作用发挥抗癫痫作用。
