Feng Q, Davey KG, Ladd TR, Retnakaran A, Tomkins BL, Zheng S, Palli SR
Great Lakes Forestry Centre, Canadian Forest Service, P.O. Box 490, 1219 Queen Street East, Ontario, P6A 5M7, Sault Ste. Marie, Canada
J Insect Physiol. 2001 Jan 1;47(1):1-10. doi: 10.1016/s0022-1910(00)00093-7.
Developmental and stress-induced expression of Choristoneura fumiferana glutathione S-transferase (CfGST) mRNA and protein were examined using Northern blots and Western blots. High levels of CfGST mRNA and protein were detected in 1st instar larvae and diapausing 2nd instar larvae. Expression of CfGST gradually decreased during larval development from 3rd to 5th instar, after which the expression increased once again, reaching peak levels in 6th instar larvae. CfGST mRNA and protein were undetectable in the pupal stage. Exposure to low temperature did not induce an increase in CfGST expression. Feeding on balsam fir foliage resulted in an increase in the expression of CfGST as compared to larvae that fed on artificial diet. The bacterial insecticide, Bacillus thuringiensis delta-endotoxin (Bt), the non-steroidal ecdysone analog, tebufenozide, and the synthetic pyrethroid, permethrin, induced the expression of CfGST mRNA in 5th instar larvae, whereas the chitin synthesis inhibitor, diflubenzuron, did not have any such effect. These results suggest that CfGST plays an important role in detoxifying various allelochemicals and insecticides in the spruce budworm. The developmental expression pattern strongly suggests that in addition to detoxification, CfGST might be involved in other functions.
利用Northern印迹法和Western印迹法检测了云杉芽卷叶蛾谷胱甘肽S-转移酶（CfGST）mRNA和蛋白质的发育表达及应激诱导表达。在1龄幼虫和滞育的2龄幼虫中检测到高水平的CfGST mRNA和蛋白质。CfGST的表达在幼虫从3龄到5龄的发育过程中逐渐下降，之后表达再次增加，在6龄幼虫中达到峰值水平。在蛹期未检测到CfGST mRNA和蛋白质。暴露于低温并未诱导CfGST表达增加。与以人工饲料为食的幼虫相比，取食香脂冷杉叶片导致CfGST表达增加。细菌杀虫剂苏云金芽孢杆菌δ-内毒素（Bt）、非甾体蜕皮激素类似物虫酰肼和拟除虫菊酯氯菊酯诱导5龄幼虫中CfGST mRNA的表达，而几丁质合成抑制剂除虫脲则没有这种作用。这些结果表明，CfGST在云杉芽卷叶蛾对各种化感物质和杀虫剂的解毒过程中起重要作用。发育表达模式强烈表明，除了解毒作用外，CfGST可能还参与其他功能。
