Tang Fang, Tu Huizhen, Shang Qingli, Gao Xiwu, Liang Pei
Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing 210037, China.
College of Forestry, Nanjing Forestry University, Nanjing 210037, China.
Insects. 2020 Jun 1;11(6):339. doi: 10.3390/insects11060339.
Plants accumulate phenolic compounds such as tannic acid to resist insect herbivores. The survival of insects exposed to toxic secondary metabolites depends on the detoxification metabolism mediated by limited groups of glutathione S-transferases (GSTs). (Graeser) (Lepidoptera: Notodontidae) is an important foliar pest of poplar trees. GSTs play an important role in xenobiotic detoxification in . Five GST genes were identified in and were classified into five different cytosolic GST classes, delta, omega, sigma, theta, and zeta. Real-time fluorescent quantitative polymerase chain reaction (qPCR) was used to determine the mRNA expression of the five cloned GSTs in the midguts and fat bodies of . The mRNA expression of the five GSTs was significantly induced when was exposed to tannic acid. To further understand the tannic acid regulatory cascade, the 5'-flanking promoter sequences of the five s were isolated by genome walking methods, and the promoters were very active and induced by tannic acid. In summary, the induction of GST mRNA expression was due to the response of five promoters to tannic acid. Therefore, promoters play an important role in the regulation of GST transcription.
植物积累酚类化合物如单宁酸以抵御食草昆虫。暴露于有毒次生代谢产物的昆虫的存活取决于由有限数量的谷胱甘肽S-转移酶（GSTs）介导的解毒代谢。格氏冬尺蛾（鳞翅目：舟蛾科）是杨树的一种重要食叶害虫。GSTs在格氏冬尺蛾的外源物解毒中起重要作用。在格氏冬尺蛾中鉴定出五个GST基因，并将其分为五个不同的胞质GST类别，即δ、ω、σ、θ和ζ。使用实时荧光定量聚合酶链反应（qPCR）来测定这五个克隆的GSTs在格氏冬尺蛾中肠和脂肪体中的mRNA表达。当格氏冬尺蛾暴露于单宁酸时，这五个GSTs的mRNA表达被显著诱导。为了进一步了解单宁酸调控级联反应，通过基因组步移法分离出这五个格氏冬尺蛾基因的5'侧翼启动子序列，并且这些启动子非常活跃且受单宁酸诱导。总之，GST mRNA表达的诱导是由于五个格氏冬尺蛾基因启动子对单宁酸的响应。因此，格氏冬尺蛾基因启动子在GST转录调控中起重要作用。
