Breslin W J, Marty M S, Vedula U V, Liberacki A B, Yano B L
Eli Lilly & Company, Indiana, Greenfield, USA.
Food Chem Toxicol. 2000 Dec;38(12):1103-12. doi: 10.1016/s0278-6915(00)00108-3.
The insecticide Spinosad was administered by gavage to pregnant CD(R) rats at 0, 10, 50 or 200 mg/kg/day on gestation days (gd) 6-15 and to New Zealand White rabbits at 0, 2.5, 10 or 50 mg/kg/day on gd-7-19. Rats and rabbits were monitored for clinical signs of toxicity and body weight gains. At gd-21 (rats) or gd-28 (rabbits), maternal organ weights, reproductive parameters, fetal body weights, and fetal external, visceral and skeletal structures were evaluated. Rats given 200 mg/kg/day exhibited a 4% lower body weight on gd-12 and decreased body weight gains on gd-6-16 relative to controls. There was no maternal toxicity at 10 or 50 mg/kg/day, and no developmental toxicity in rats at any dose level. Rabbits given 50 mg/kg/day exhibited decreased feed consumption, reduced fecal output, body weight loss during the initial dosing period (gd-7-10) and a non-statistically significant decrease (31%) in body weight gain during the dosing period (gd-7-20). Two litters aborted due to maternal inanition. There were no maternal effects at lower doses, and no signs of developmental toxicity at any dose. Thus, the maternal no-observed-effect levels (NOEL) were 50 and 10 mg/kg/day in rats and rabbits, respectively, and the embryonal/fetal NOELs were 200 mg/kg/day in rats and 50 mg/kg/day in rabbits.
在妊娠第6至15天，通过灌胃法给妊娠的CD（R）大鼠分别施以0、10、50或200毫克/千克/天的杀虫剂多杀菌素；在妊娠第7至19天，给新西兰白兔分别施以0、2.5、10或50毫克/千克/天的该杀虫剂。监测大鼠和兔子的毒性临床体征及体重增加情况。在妊娠第21天（大鼠）或第28天（兔子），评估母体器官重量、生殖参数、胎儿体重以及胎儿的外部、内脏和骨骼结构。与对照组相比，给予200毫克/千克/天的大鼠在妊娠第12天体重低4%，且在妊娠第6至16天体重增加减少。给予10或50毫克/千克/天未出现母体毒性，且在任何剂量水平下大鼠均未出现发育毒性。给予50毫克/千克/天的兔子出现摄食量减少、粪便排出量减少、在初始给药期（妊娠第7至10天）体重减轻，以及在给药期（妊娠第7至20天）体重增加有非统计学意义的减少（31%）。两窝幼崽因母体营养缺乏而流产。较低剂量时未出现母体影响，且在任何剂量下均未出现发育毒性迹象。因此，大鼠和兔子的母体未观察到效应水平（NOEL）分别为50和10毫克/千克/天，大鼠和兔子的胚胎/胎儿NOEL分别为200毫克/千克/天和50毫克/千克/天。
