Gharagozloo S, Sharifian R A, Mageed R A, Shokri F
School of Public Health Tehran University of Medical Sciences, Department of Immunology, Tehran, 14155, I.R. Iran.
Pathol Oncol Res. 2000;6(3):185-90. doi: 10.1007/BF03032371.
The frequency of expression of immunoglobulin (Ig) variable region heavy (VH ) chain gene products was studied in 43 Iranian patients with mutiple myeloma (MM). The expressed VH gene families and associated cross-reactive idiotypes (CRI) were analysed by immunoblotting and ELISA, using peptide-induced polyclonal antibodies specific for VH 1-VH 6 gene families and monoclonal antibodies (MAb) recognising CRI linked to theVH 1, VH 3, VH 4 and VH 6 gene families. The results revealed that the VH 3 family (60. 5%) was the most predominant gene family. In contrast, no paraproteins were encoded by genes from the VH 2 gene family and only 2.3% were encoded by the VH 5 family. The panel of paraproteins tested rarely expressed the probed VH -associated CRI. Our results suggest that: 1-The Ig VH genes, may not be randomly expressed in the malignant plasma cells from Iranian patients with MM. 2- Some of the genes seem to be negatively selected or highly mutated, as evidenced by the lack of expression of the probed CRI.
对43例伊朗多发性骨髓瘤（MM）患者免疫球蛋白（Ig）可变区重链（VH）基因产物的表达频率进行了研究。使用针对VH 1-VH 6基因家族的肽诱导多克隆抗体以及识别与VH 1、VH 3、VH 4和VH 6基因家族相关的交叉反应性独特型（CRI）的单克隆抗体（MAb），通过免疫印迹和酶联免疫吸附测定（ELISA）分析表达的VH基因家族和相关的交叉反应性独特型。结果显示，VH 3家族（60.5%）是最主要的基因家族。相比之下，VH 2基因家族的基因未编码副蛋白，VH 5家族仅编码2.3%的副蛋白。所检测的副蛋白很少表达所探测的与VH相关的CRI。我们的结果表明：1-Ig VH基因在伊朗MM患者的恶性浆细胞中可能并非随机表达。2-一些基因似乎被阴性选择或高度突变，这从所探测的CRI缺乏表达得到证明。
