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[利用等位酶标记评估堪察加拟石蟹(Paralithodes camtschaticus (Tilesius))的遗传变异性]

[Evaluation of genetic variability of the Kamchatka crab Paralithodes camtschaticus (Tilesius) using allozyme markers].

作者信息

Balakirev E S, Fedoseev V Ia

机构信息

Institute of Marine Biology, Russian Academy of Sciences, Vladivostok, Russia.

出版信息

Genetika. 2000 Aug;36(8):1041-8.

Abstract

Interspecific genetic variation in populations of red king crab Paralithodes camtschaticus Tilesius (Litholidae, Decapoda: Crustacea) was examined using allozyme markers. The activity of 57 enzymes and the general protein presumably encoded by 92 loci was detected. The level of allozyme variability was low: the expected heterozygosity and the proportion of polymorphic loci were respectively 0.027 +/- 0.008 and 6.5%. This level of heterozygosity is three times lower than the average value for 122 crustacean species (0.082 +/- 0.007). Although genetic variants were found at 22 loci, their frequencies were generally low: only in loci 6-Pgd, Alp-1, and Pep-1 did the frequencies of the most common alleles not exceed 0.9. All polymorphic loci except one had two alleles; the exception was 6-Pgd, which had three alleles. The possible reasons for the low level of allozyme variability in red king crab are discussed.

摘要

利用等位酶标记对红帝王蟹(Paralithodes camtschaticus Tilesius,石蟹科,十足目:甲壳纲)种群间的种间遗传变异进行了研究。检测了57种酶的活性以及可能由92个基因座编码的总蛋白。等位酶变异性水平较低:预期杂合度和多态性基因座比例分别为0.027±0.008和6.5%。这种杂合度水平比122种甲壳类动物的平均值(0.082±0.007)低三倍。尽管在22个基因座上发现了遗传变异,但其频率通常较低:只有在6-Pgd、Alp-1和Pep-1基因座上,最常见等位基因的频率不超过0.9。除一个基因座外,所有多态性基因座都有两个等位基因;例外的是6-Pgd,它有三个等位基因。文中讨论了红帝王蟹等位酶变异性水平低的可能原因。

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