Bogdanenko E V, Sviridov Iu V, Moskovtsev A A, Zhdanov R I
Research Institute for Biomedical Chemistry, RAMS, Moscow.
Vopr Med Khim. 2000 May-Jun;46(3):226-45.
Recent findings connected with in vivo use of artificial macromolecular complexes (genosomes) for functional gene transfer and delivery are discussed in the paper. Non-viral methods are the most safe for the purpose of human gene delivery. The cationic liposomes containing cholesterol are the most suitable for this purpose, because they possess high biodegradability and stability in blood stream. The DNA-liposome complexes should: (i) contain DNA in the condition at most protected from environmental influence, (ii) be rather homogeneous and of small size (40-80 nm). Injections of complexes into blood are the most effective; in a respect of organospecifity may be achieved by appropriate ligand selection. It is the most perspectively to increase the expression level by combining liposomes with viral peptides.
本文讨论了与人工大分子复合物（基因体）在体内用于功能性基因转移和递送相关的最新研究结果。非病毒方法对于人类基因递送而言是最安全的。含胆固醇的阳离子脂质体最适合此目的，因为它们在血流中具有高生物降解性和稳定性。DNA-脂质体复合物应：（i）在最能免受环境影响的条件下包含DNA，（ii）相当均匀且尺寸小（40-80纳米）。将复合物注入血液是最有效的；在器官特异性方面可通过适当选择配体来实现。将脂质体与病毒肽结合以提高表达水平是最具前景的。
