Sheikhi M, Gustafsson A, Jarstrand C
Division of Clinical and Oral Bacteriology, Karolinska Institute, Huddinge Hospital, Sweden.
J Clin Periodontol. 2000 Oct;27(10):758-62. doi: 10.1034/j.1600-051x.2000.027010758.x.
Periodontitis is characterised by tissue destruction caused by reactive oxygen species (ROS) and proteolytic enzymes, which are released by the interaction between bacteria and phagocytes. We estimated the ability of Fusobacterium species to induce release of tissue destructive and proinflammatory mediators from in vitro activated peripheral leukocytes. ROS was measured with the nitroblue tetrazolium (NBT) method, elastase with a specific chromogenic substrate and cytokines, including interleukin 1beta (IL-1beta), tumour necrosis factor alpha (TNF-alpha), and interleukin 8 (IL-8) with a sandwich ELISA method. Various clinical isolates of unopsonized Fusobacterium species stimulated the neutrophils to an increased NBT- reduction. IL-1beta, TNFalpha, IL-8 and elastase were released in significantly higher levels from neutrophils stimulated by Fusobacterium species. In conclusion, unopsonized Fusobacterium species can induce increased production of oxygen radicals, cytokines and elastase from leukocytes activated in vitro.
牙周炎的特征是由活性氧（ROS）和蛋白水解酶引起的组织破坏，这些物质是细菌与吞噬细胞相互作用释放出来的。我们评估了梭杆菌属物种诱导体外激活的外周血白细胞释放组织破坏性和促炎介质的能力。采用硝基蓝四氮唑（NBT）法测定ROS，用特异性显色底物测定弹性蛋白酶，并用夹心ELISA法测定细胞因子，包括白细胞介素1β（IL-1β）、肿瘤坏死因子α（TNF-α）和白细胞介素8（IL-8）。各种未调理的梭杆菌属临床分离株刺激中性粒细胞增加NBT还原。梭杆菌属物种刺激的中性粒细胞释放的IL-1β、TNFα、IL-8和弹性蛋白酶水平显著更高。总之，未调理的梭杆菌属物种可诱导体外激活的白细胞增加氧自由基、细胞因子和弹性蛋白酶的产生。
