Rojas P A, Martin V, Nigro M, Echeverria P C, Guarnera E A, Pszenny V, Angel S O
Departamento de Parasitología Sanitaria, Instituto Nacional de Parasitología, Buenos Aires, Argentina.
FEMS Microbiol Lett. 2000 Sep 15;190(2):209-13. doi: 10.1111/j.1574-6968.2000.tb09288.x.
A cDNA clone (Tgzy85d11.r1) obtained from the Toxoplasma Expressed Sequence Tag project was chosen due to its homology with proteins of the heat shock 90 family. The cDNA encodes 137 amino acids of the C-terminal portion of the Toxoplasma Hsp90 protein (TgHsp90). Serum samples obtained from orally infected BALB/c and C57BL/6 mice showed reactivity against a recombinant TgHsp90 (rTgHsp90) after 8 weeks postinfection. Isotype analysis showed an anti-rTgHsp90 IgG2a/IgG3 response in infected BALB/c and anti-rTgHsp90 IgG1/IgG2a/IgG2b response in infected C57BL/6 mice. Serum samples from individuals chronically and putative acutely infected with T. gondii showed a similar anti-rTgHsp90 IgG response. Our work identifies TgHsp90 as a novel parasite antigen that seems to elicit a higher relation of anti-TgHsp90/anti-T. gondii IgGs during chronic infection in comparison with the acute stage.
从弓形虫表达序列标签项目中获得的一个cDNA克隆（Tgzy85d11.r1）因其与热休克90家族蛋白的同源性而被选中。该cDNA编码弓形虫热休克蛋白90（TgHsp90）C端部分的137个氨基酸。从经口感染的BALB/c和C57BL/6小鼠获得的血清样本在感染后8周显示出对重组TgHsp90（rTgHsp90）的反应性。亚型分析显示，感染的BALB/c小鼠出现抗rTgHsp90 IgG2a/IgG3反应，感染的C57BL/6小鼠出现抗rTgHsp90 IgG1/IgG2a/IgGb反应。来自慢性和疑似急性感染弓形虫个体的血清样本显示出类似的抗rTgHsp90 IgG反应。我们的研究确定TgHsp90是一种新型寄生虫抗原，与急性期相比，在慢性感染期间似乎能引发更高比例的抗TgHsp90/抗弓形虫IgG。
