Gioppo N M, Elias W P, Vidotto M C, Linhares R E, Saridakis H O, Gomes T A, Trabulsi L R, Pelayo J S
Departamento de Microbiologia, Universidade Estadual de Londrina, Brazil.
FEMS Microbiol Lett. 2000 Sep 15;190(2):293-8. doi: 10.1111/j.1574-6968.2000.tb09301.x.
A total of 919 Escherichia coli isolates from 125 children with diarrhoea (cases) and 98 controls were assayed for adherence to HEp-2 cells. Localised adherence was found only in isolates from cases. Diffuse, aggregative (AA), chain-like adherence (CLA) and variants of the AA pattern were found in both cases and controls. The AA isolates were tested for gene sequences associated with enteroaggregative E. coli (EAEC). Only 25% of the isolates hybridised with the EAEC probe, and the aafA, astA and pet gene sequences were found in 7.9%, 44.7% and 7.9% of the isolates, respectively. The aggA gene was not found, although 7.9% were positive for aggC. The CLA isolates reacted with the EAEC probe (55.6%), and the aggC, astA and pet gene sequences were found in 66.7%, 33.3% and 11.1%, respectively. The aggR (55.6%), aspU (55.6%), shf (33.3%) and she (22.2%) genes were also found in CLA isolates.
对125例腹泻儿童（病例）和98例对照的919株大肠杆菌分离株进行了对人喉上皮癌细胞（HEp-2细胞）黏附性的检测。仅在病例的分离株中发现了局限性黏附。在病例和对照中均发现了弥漫性、聚集性（AA）、链状黏附（CLA）以及AA模式的变体。对AA分离株进行了与肠聚集性大肠杆菌（EAEC）相关的基因序列检测。只有25%的分离株与EAEC探针杂交，分别有7.9%、44.7%和7.9%的分离株中发现了aafA、astA和pet基因序列。未发现aggA基因，不过有7.9%的分离株aggC呈阳性。CLA分离株与EAEC探针发生反应（55.6%），分别有66.7%、33.3%和11.1%的分离株中发现了aggC、astA和pet基因序列。在CLA分离株中还发现了aggR（55.6%）、aspU（55.6%）、shf（33.3%）和she（22.2%）基因。
