Weiner H, Kaiser W M
Julius-von-Sachs-Institut für Biowissenschaften, Lehrstuhl Botanik I, Molekulare Pflanzenphysiologie und Biophysik, Würzburg, Germany.
FEBS Lett. 2000 Sep 1;480(2-3):217-20. doi: 10.1016/s0014-5793(00)01940-2.
To assess the role of 14-3-3 proteins in the magnesium-dependent inhibition of nitrate reductase (NR) we tested the effect of magnesium on NR binding to 14-3-3s by coimmunoprecipitation and gel filtration. The stability of the 14-3-3 complex of NR was, unlike its activity, unaffected by magnesium. We therefore conclude that binding to 14-3-3s per se does not inhibit NR. Magnesium inhibited 14-3-3-bound NR much more strongly than 14-3-3-free NR. 14-3-3s possibly reinforce NR inhibition by magnesium.
为评估14-3-3蛋白在镁依赖的硝酸还原酶（NR）抑制作用中的角色，我们通过免疫共沉淀和凝胶过滤测试了镁对NR与14-3-3结合的影响。与NR的活性不同，其与14-3-3形成的复合物的稳定性不受镁的影响。因此，我们得出结论，与14-3-3本身的结合并不会抑制NR。镁对与14-3-3结合的NR的抑制作用比对未与14-3-3结合的NR的抑制作用要强得多。14-3-3可能会增强镁对NR的抑制作用。
