Noordeen F, Rajapakse R P, Faizal A C, Horadagoda N U, Arulkanthan A
Department of Veterinary Paraclinical Studies, Faculty of Veterinary Medicine and Animal Science, University of Peradeniya, Peradeniya, Sri Lanka.
Vet Parasitol. 2000 Nov 10;93(2):95-101. doi: 10.1016/s0304-4017(00)00361-7.
The prevalence of Cryptosporidium oocysts in the faeces of 1020 goats in three age categories was examined during 1999 in selected locations of three agroclimatic zones of Sri Lanka. The oocysts were demonstrated using the Sheather's sucrose flotation method followed by staining with the modified Ziehl Neelsen technique. Cryptosporidium oocysts were detected in animals from all agroclimatic zones with the highest prevalence of infection in the dry zone (33.6%) compared with 24.7 and 21.7% in the intermediate zones and wet, respectively (P<0.001). Overall, Cryptosporidium oocyst counts were significantly higher in goats of <6 months and 7-12 months of age groups compared with goats of >12 months of age (P<0.001). Cryptosporidium oocysts were detected in 291/1020 (28.5%) animals, while 194/1020 animals (19%), 84/1020 animals (8.2%) and 13/1020 animals (1.3%) excreted low (1-1000 oocysts per gram of faeces), moderate (1000-5000 oocysts per gram of faeces) and high (>5000 oocysts per gram of faeces) counts, respectively. The mean Cryptosporidium count was 383 oocysts per gram of faeces. The majority of the infected goats were asymptomatic. These animals are likely to play an important role in the epidemiology of cryptosporidiosis in goat kids and humans.
1999年，在斯里兰卡三个农业气候区的选定地点，对1020只三个年龄组的山羊粪便中的隐孢子虫卵囊流行情况进行了检查。采用Sheather蔗糖漂浮法检测卵囊，随后用改良的齐-尼氏技术染色。在所有农业气候区的动物中均检测到隐孢子虫卵囊，干旱区的感染率最高（33.6%），而中间区和湿润区分别为24.7%和21.7%（P<0.001）。总体而言，6个月龄以下和7-12个月龄组的山羊隐孢子虫卵囊计数显著高于12个月龄以上的山羊（P<0.001）。在1020只动物中有291只（28.5%）检测到隐孢子虫卵囊，而194只（19%）、84只（8.2%）和13只（1.3%）动物分别排出低（每克粪便1-1000个卵囊）、中（每克粪便1000-5000个卵囊）和高（每克粪便>5000个卵囊）计数的卵囊。隐孢子虫的平均计数为每克粪便383个卵囊。大多数受感染的山羊没有症状。这些动物可能在山羊幼崽和人类隐孢子虫病的流行病学中起重要作用。
