Chartier Christophe, Mallereau-Pellet Marie Pierre, Mancassola Roselyne, Nussbaum David
AFSSA-Niort, Laboratoire d'Etudes et de Recherches Caprines, France.
Vet Res. 2002 Mar-Apr;33(2):169-77. doi: 10.1051/vetres:2002005.
Detection of Cryptosporidium oocysts from goat kid faeces: comparison of a latex agglutination test with three other conventional techniques. A quantitative latex agglutination test (QLAT) with monoclonal antibodies for the detection of Cryptosporidium oocysts in faeces was compared with 3 other conventional techniques: Heine staining on faecal smears (HS) giving semi-quantitative results (scores from 1 to 5), sucrose flotation on diluted faeces (SF) with results expressed in oocysts/g of faeces (opg), direct ELISA (DE) giving qualitative results. Goat kid unconcentrated faecal samples (234) from 8 farms were processed according to the 4 techniques. Data were analyzed with Win Episcope 1.0 and Testview 1.1 softwares. The oocyst outputs ranged from 100 000 (detection limit for SF) to 200 millions opg (mean: 15.2 millions opg). A very good agreement was recorded between QLAT and HS, SF, DE: Kappa values ranged between 0.82 and 0.90. When considering the samples exhibiting oocysts (or not) as positive (or negative) using both HS and SF (n = 219), the sensitivity and specificity of QLAT were respectively 95.1 and 96.0%. The lack of sensitivity was observed in faeces harboring a few oocysts (< or = 200 000 opg, scores < or = 2) whereas the lack of specificity was only observed in 3 samples originating from the same farm. A significant correlation was calculated between the percentage of agglutination in QLAT and the number of oocysts in SF or scores in HS (Spearman correlation ranging from 0.45 to 0.48, p < 0.001). QLAT is a rapid, simple and reliable tool for routine detection of Cryptosporidium oocysts in faeces.
乳胶凝集试验与其他三种传统技术的比较。将一种用于检测粪便中隐孢子虫卵囊的单克隆抗体定量乳胶凝集试验（QLAT）与其他三种传统技术进行比较：粪便涂片的海涅染色（HS）给出半定量结果（评分从1到5）、稀释粪便的蔗糖漂浮法（SF）结果以每克粪便中的卵囊数（opg）表示、直接酶联免疫吸附测定（DE）给出定性结果。对来自8个农场的234份未浓缩的山羊羔粪便样本按照这4种技术进行处理。数据用Win Episcope 1.0和Testview 1.1软件进行分析。卵囊产量范围从100000（SF的检测限）到2亿opg（平均值：1520万opg）。QLAT与HS、SF、DE之间记录到非常好的一致性：卡帕值在0.82至0.90之间。当使用HS和SF将显示（或未显示）卵囊的样本视为阳性（或阴性）时（n = 219），QLAT的灵敏度和特异性分别为95.1%和96.0%。在含有少量卵囊（≤200000 opg，评分≤2）的粪便中观察到灵敏度不足，而仅在来自同一农场的3个样本中观察到特异性不足。计算出QLAT中的凝集百分比与SF中的卵囊数或HS中的评分之间存在显著相关性（斯皮尔曼相关性范围从0.45至0.48，p < 0.001）。QLAT是粪便中隐孢子虫卵囊常规检测的一种快速、简单且可靠的工具。
