有袋类动物前列腺的形成是由睾丸雄激素5α-雄烷-3α,17β-二醇介导的。
Prostate formation in a marsupial is mediated by the testicular androgen 5 alpha-androstane-3 alpha,17 beta-diol.
作者信息
Shaw G, Renfree M B, Leihy M W, Shackleton C H, Roitman E, Wilson J D
机构信息
Department of Zoology, University of Melbourne, Victoria 3010, Australia.
出版信息
Proc Natl Acad Sci U S A. 2000 Oct 24;97(22):12256-9. doi: 10.1073/pnas.220412297.
Abstract
Development of the male urogenital tract in mammals is mediated by testicular androgens. It has been tacitly assumed that testosterone acts through its intracellular metabolite dihydrotestosterone (DHT) to mediate this process, but levels of these androgens are not sexually dimorphic in plasma at the time of prostate development. Here we show that the 3 alpha-reduced derivative of DHT, 5 alpha-androstane-3 alpha,17 beta-diol (5 alpha-adiol), is formed in testes of tammar wallaby pouch young and is higher in male than in female plasma in this species during early sexual differentiation. Administration of 5 alpha-adiol caused formation of prostatic buds in female wallaby pouch young, and in tissue minces of urogenital sinus and urogenital tubercle radioactive 5 alpha-adiol was converted to DHT, suggesting that circulating 5 alpha-adiol acts through DHT in target tissues. We conclude that circulating 5 alpha-adiol is a key hormone in male development.
摘要
哺乳动物雄性泌尿生殖道的发育由睾丸雄激素介导。一直以来人们默认睾酮通过其细胞内代谢产物双氢睾酮（DHT）来介导这一过程，但在前列腺发育时，这些雄激素在血浆中的水平并无性别差异。在此我们表明，DHT的3α-还原衍生物，5α-雄烷-3α,17β-二醇（5α-二醇），在袋鼠幼崽的睾丸中形成，并且在该物种早期性别分化期间，雄性血浆中的5α-二醇水平高于雌性。给雌性袋鼠幼崽注射5α-二醇会导致前列腺芽的形成，并且在泌尿生殖窦和泌尿生殖结节的组织碎块中，放射性5α-二醇会转化为DHT，这表明循环中的5α-二醇在靶组织中通过DHT发挥作用。我们得出结论，循环中的5α-二醇是雄性发育中的关键激素。
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本文引用的文献
1
Virilization of the male pouch young of the tammar wallaby does not appear to be mediated by plasma testosterone or dihydrotestosterone.帚尾袋鼩雄性育儿袋幼崽的雄性化似乎并非由血浆睾酮或双氢睾酮介导。
Biol Reprod. 1999 Aug;61(2):471-5. doi: 10.1095/biolreprod61.2.471.
2
Development of the penis and clitoris in the tammar wallaby, Macropus eugenii.帚尾袋鼩（Macropus eugenii）阴茎与阴蒂的发育
Anat Embryol (Berl). 1999 May;199(5):451-7. doi: 10.1007/s004290050243.
3
Postnatal development of Leydig cells in the opossum (Monodelphis domestica): an immunocytochemical and endocrinological study.负鼠（Monodelphis domestica）睾丸间质细胞的产后发育：一项免疫细胞化学和内分泌学研究。
Biol Reprod. 1998 Mar;58(3):664-9. doi: 10.1095/biolreprod58.3.664.
4
Sex hormone-binding globulin mediates prostate androgen receptor action via a novel signaling pathway.性激素结合球蛋白通过一条新的信号通路介导前列腺雄激素受体的作用。
Endocrinology. 1998 Jan;139(1):213-8. doi: 10.1210/endo.139.1.5681.
5
Molecular endocrinology of hydroxysteroid dehydrogenases.羟类固醇脱氢酶的分子内分泌学
Endocr Rev. 1997 Jun;18(3):281-305. doi: 10.1210/edrv.18.3.0302.
6
The influence of the anti-androgen flutamide on early sexual differentiation of the marsupial male.抗雄激素氟他胺对有袋类雄性动物早期性分化的影响。
J Reprod Fertil. 1997 Mar;109(2):205-12. doi: 10.1530/jrf.0.1090205.
7
Effects of a 5 alpha-reductase inhibitor, finasteride, on the developing prostate and testis of a marsupial.5α-还原酶抑制剂非那雄胺对有袋动物发育中的前列腺和睾丸的影响。
J Androl. 1997 Mar-Apr;18(2):123-30.
8
Confirming testosterone administration by isotope ratio mass spectrometric analysis of urinary androstanediols.通过尿雄烷二醇的同位素比率质谱分析确认睾酮给药情况。
Steroids. 1997 Apr;62(4):379-87. doi: 10.1016/s0039-128x(96)00253-x.
9
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Behav Brain Res. 1996 Sep;79(1-2):109-18. doi: 10.1016/0166-4328(96)00004-6.
10
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