Lippincott J, Li R
Department of Cell Biology, Harvard Medical School, 240 Longwood Avenue, Boston, Massachusetts, 02115, USA.
Exp Cell Res. 2000 Nov 1;260(2):277-83. doi: 10.1006/excr.2000.5021.
We have investigated the relationship between nuclear envelope fission and cytokinesis during mitotic cell division in budding yeast. By carrying out time-lapse and optical sectioning video microscopy analysis of cells that express green fluorescent protein (GFP)-tagged nuclear envelope and actomyosin ring components, we found that nuclear division is temporally coupled to cytokinesis. Light and electron microscopy analysis also showed that nuclear envelope fission and the division of the nucleoplasm are severely delayed in cytokinesis mutants, resulting in discoupling between the nuclear division cycle and the budding cycle. These results suggest that homotypic membrane fusion may be activated by components or the mechanical action of cytokinetic structures and presents a mechanism for the equal partitioning of the nucleus and the temporal coordination of this event with chromosome segregation during mitosis.
我们研究了出芽酵母有丝分裂细胞分裂过程中核膜裂变与胞质分裂之间的关系。通过对表达绿色荧光蛋白（GFP）标记的核膜和肌动球蛋白环成分的细胞进行延时和光学切片视频显微镜分析，我们发现核分裂在时间上与胞质分裂相关联。光镜和电镜分析还表明，在胞质分裂突变体中，核膜裂变和核质分裂严重延迟，导致核分裂周期与出芽周期解偶联。这些结果表明，同型膜融合可能由胞质分裂结构的成分或机械作用激活，并提出了一种在有丝分裂期间细胞核均等分配以及该事件与染色体分离时间协调的机制。
