Gola S, Hegner J, Kothe E
Mikrobiology-Microbial Phytopathology, Friedrich-Schiller-University, Beutenbergstr. 11, 07745 Jena, Germany.
Fungal Genet Biol. 2000 Aug;30(3):191-6. doi: 10.1006/fgbi.2000.1222.
The pheromone receptor system of the basidiomycete Schizophyllum commune is capable of ligand discrimination to confer mating specificity. The pheromone receptors of the B alpha locus were investigated for ligand discrimination in a strategy of domain swapping experiments. Several altered phenotypes of chimeric receptors have been found. These include constitutive pheromone receptors which need no ligand for activation of the downstream cascade of events. In addition, receptors still dependent on ligand were identified that had altered pheromone activation profiles, including promiscuous receptors that are activated by pheromones of all nine specificities, including the former self. In addition, highly discriminative receptors were created which are activated by only two of the eight non-self-specificities. The chimeric receptors identify the last third of the receptor as the determinant for B alpha 1 specificity, whereas B alpha 2 specificity resides in noncontiguous domains covering the first and middle parts of the receptor molecule.
担子菌裂褶菌的信息素受体系统能够进行配体识别以赋予交配特异性。在结构域交换实验策略中，对Bα位点的信息素受体进行了配体识别研究。已发现嵌合受体的几种改变的表型。这些包括组成型信息素受体，其不需要配体来激活下游事件级联反应。此外，还鉴定出仍依赖配体的受体，其具有改变的信息素激活谱，包括被所有九种特异性的信息素激活的混杂受体，包括以前的自身信息素。此外，还构建了高度特异性的受体，其仅被八种非自身特异性中的两种激活。嵌合受体将受体的最后三分之一识别为Bα1特异性的决定因素，而Bα2特异性存在于覆盖受体分子第一和中间部分的不连续结构域中。
