Wendland J, Vaillancourt L J, Hegner J, Lengeler K B, Laddison K J, Specht C A, Raper C A, Kothe E
Philipps-Universität, Fachbereich Biologie-Molekulargenetik, Marburg, Germany.
EMBO J. 1995 Nov 1;14(21):5271-8. doi: 10.1002/j.1460-2075.1995.tb00211.x.
Analysis of the multispecific B alpha mating-type locus of Schizophyllum commune provided evidence that pheromones and pheromone receptors govern recognition of self versus non-self and sexual development in this homobasidiomycetous fungus. Four subclones of an 8.2 kb genomic fragment carrying B alpha 1 specificity induced B-regulated sexual morphogenesis when introduced into a strain with one of the eight compatible B alpha specificities that are known to exist in nature. One of these clones, which activated all other B alpha specificities, contains a gene termed bar1. The predicted protein product of bar1, as well as that of bar2, a homologous gene isolated from a B alpha 2 strain, has significant homology to known fungal pheromone receptor proteins in the rhodopsin-like superfamily of G protein-linked receptors. The other three active B alpha 1 clones were subcloned further to identify the minimal active element in each clone. Every active subclone contains a putative pheromone gene ending in a signal for possible isoprenylation. A message of approximately 600 bp was observed for one of these genes, bap1(1). This paper presents the first evidence for a system of multiple pheromones and pheromone receptors as a basis for multispecific mating types in a fungus.
裂褶菌多特异性Bα交配型位点的分析提供了证据，表明信息素和信息素受体在这种同担子菌纲真菌中决定了自我与非自我的识别以及有性发育。携带Bα1特异性的8.2 kb基因组片段的四个亚克隆，当被导入到一个具有自然界已知存在的八种兼容Bα特异性之一的菌株中时，诱导了B调节的有性形态发生。其中一个激活了所有其他Bα特异性的克隆包含一个名为bar1的基因。bar1的预测蛋白质产物以及从Bα2菌株中分离出的同源基因bar2的产物，与G蛋白偶联受体的视紫红质样超家族中已知的真菌信息素受体蛋白具有显著的同源性。对另外三个有活性的Bα1克隆进行了进一步亚克隆，以确定每个克隆中的最小活性元件。每个有活性的亚克隆都包含一个推定的信息素基因，其末端有一个可能用于异戊二烯化的信号。其中一个基因bap1(1)观察到约600 bp的转录本。本文首次提供了一个证据，证明在一种真菌中存在一个由多种信息素和信息素受体组成的系统，作为多特异性交配型的基础。
