Purification of proteins using polyhistidine affinity tags.
作者信息
Bornhorst J A, Falke J J
机构信息
Department of Chemistry and Biochemistry, University of Colorado, Boulder 80309-0215, USA.
出版信息
Methods Enzymol. 2000;326:245-54. doi: 10.1016/s0076-6879(00)26058-8.
Abstract
摘要
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Immobilized metal ion affinity chromatography on Co2+-carboxymethylaspartate-agarose Superflow, as demonstrated by one-step purification of lactate dehydrogenase from chicken breast muscle.
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