使用6xHis标签和Ni-NTA树脂一步纯化重组蛋白。
One-step purification of recombinant proteins with the 6xHis tag and Ni-NTA resin.
作者信息
Crowe J, Masone B S, Ribbe J
机构信息
QIAGEN Inc., Chatsworth, CA 91311, USA.
出版信息
Mol Biotechnol. 1995 Dec;4(3):247-58. doi: 10.1007/BF02779018.
PMID:8680931
Abstract
The 6xHis/Ni-NTA system allows rapid and efficient affinity purification of recombinant proteins from virtually any expression system. Protocols and tips for purification under both native and denaturing conditions are provided, as well as a rapid spin procedure for protein minipreps.
摘要
6xHis/Ni-NTA系统能够从几乎任何表达系统中快速高效地亲和纯化重组蛋白。本文提供了在天然和变性条件下进行纯化的方案与技巧，以及用于蛋白质微量制备的快速离心方法。
Zotero 插件