Stepanov I I, Losev N A
Department of Neurophysiology, Institute of Experimental Medicine, Russian Academy of Medical Sciences, St. Petersburg.
Neurosci Behav Physiol. 2000 Sep-Oct;30(5):559-63. doi: 10.1007/BF02462615.
The characteristics of cholinoreceptors located on neurons TAN1, TAN2, and TAN3 of the ground snail Achatina fulica were studied by incubation of the central ganglia in a bath with cholinotropic preparations during intracellular recording of background neuron spike activity. Acetylcholine, nicotine, the selective n-cholinoreceptor agonist suberyldicholine, and the selective n-cholinoreceptor agonist 5-methylfurmethide concentration-dependently inhibited background spike activity to the level of complete blockade at concentrations of 500 microM. The m-cholinoblocker metamizil (500 microM) completely prevented the inhibitory activity of concentrations of 5-methylfurmethide of up to 500 microM. The central n-cholinoblocker etherophen (500 microM) completely blocked the inhibitory activity of 500 microM suberyldicholine. However, metamizil and etherophen added separately only partially decreased the inhibitory effects of acetylcholine but could not completely block the effect of acetylcholine. At the same time, mixtures of metamizil and etherophen (500 microM each) completely blocked the inhibition of background spike activity induced by acetylcholine. These results show that both classes of cholinoreceptors act on TAN neurons in the same direction.
通过在细胞内记录背景神经元放电活动期间，将中枢神经节置于含有拟胆碱制剂的浴槽中孵育，研究了非洲大蜗牛神经元TAN1、TAN2和TAN3上胆碱能受体的特性。乙酰胆碱、尼古丁、选择性N胆碱能受体激动剂辛二酰胆碱和选择性N胆碱能受体激动剂5-甲基糠甲铵浓度依赖性地抑制背景放电活动，在浓度为500微摩尔时抑制至完全阻断水平。M胆碱能阻滞剂安乃近（500微摩尔）完全阻止了浓度高达500微摩尔的5-甲基糠甲铵的抑制活性。中枢N胆碱能阻滞剂乙氧苯（500微摩尔）完全阻断了500微摩尔辛二酰胆碱的抑制活性。然而，单独添加安乃近和乙氧苯仅部分降低了乙酰胆碱的抑制作用，但不能完全阻断乙酰胆碱的作用。同时，安乃近和乙氧苯的混合物（各500微摩尔）完全阻断了乙酰胆碱诱导的背景放电活动的抑制。这些结果表明，两类胆碱能受体对TAN神经元的作用方向相同。
