中国分离的甜菜坏死黄脉病毒RNA5的检测与核苷酸分析
Detection and nucleotide analysis of RNA5 of beet necrotic yellow vein virus isolated in China.
作者信息
Dawei L, Jialin Y, Chenggui H, Tao L, Shucai Q, Koenig R, Yi L
机构信息
National Laboratory for Agrobiotechnology, China Agricultural University, Beijing.
出版信息
Chin J Biotechnol. 1999;15(4):253-7.
Using the RT-PCR method, five isolates of beet necrotic yellow vein virus from China were used for the RNA5 genomic component detection and sequence analysis. The result showed that RNA5 was only found in the isolates from Baotou and Hohhot, but not in those of Xingjiang, Helongjiang, and Wulate of Inner Mongolia. The RNA5 components had 1338 nucleotides and 1358 nucleotides in the length of Baotou and Hohhot isolates, respectively, in which the single open reading frame (ORF) encoding for a proteins of 26kD were contained. Compared with the published sequences of F72 and D5 isolates, these RNA5 components shared the identity of 93.7% approximately 98.5% in nucleotide acids and 91.8%-98.2% in deduced amino acids.
采用逆转录聚合酶链反应（RT-PCR）方法，对来自中国的5株甜菜坏死黄脉病毒分离物进行RNA5基因组组分检测和序列分析。结果表明，仅在包头和呼和浩特的分离物中发现了RNA5，而新疆、黑龙江和内蒙古乌拉特的分离物中未发现。包头和呼和浩特分离物的RNA5组分长度分别为1338个核苷酸和1358个核苷酸，其中包含一个编码26kD蛋白质的单一开放阅读框（ORF）。与已发表的F72和D5分离物序列相比，这些RNA5组分在核苷酸水平上的同源性约为93.7% - 98.5%，在推导氨基酸水平上的同源性为91.8% - 98.2%。
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