Yu J, Li D, Yang L, Liu Y
National Laboratory for Agrobiotechnology, Beijing Agricultural University, China.
Chin J Biotechnol. 1995;11(2):119-23.
With viral RNA extracted from purified beet necrotic yellow vein virus (BNYVV) isolated from Inner Mongolia, the first strand of cDNA encoding a 54kDa fragment of the 75kDa readthrough protein on the RNA2 was synthesized by reverse transcription. A double-strand cDNA fragment of 1.5 kb was obtained after 30 cycles of PCR amplification. The fragment was ligated into and mapped on pGEM-7Zf(+). The result of sequence analysis shows that the 54kDa readthrough domain is 1509 nucleotides (nt). Compared with F13 isolate, the fragment shares 94.97% identity in terms of nucleotides with 3 nt deletion and 96.42% identity of deduced amino acids.
从内蒙古分离得到的纯化甜菜坏死黄脉病毒（BNYVV）中提取病毒RNA，通过反转录合成了编码RNA2上75kDa通读蛋白54kDa片段的cDNA第一链。经过30个循环的PCR扩增后，获得了一个1.5kb的双链cDNA片段。该片段被连接到pGEM-7Zf(+)载体中并进行定位。序列分析结果表明，54kDa通读结构域为1509个核苷酸（nt）。与F13分离株相比，该片段在核苷酸水平上具有94.97%的同一性，有3个核苷酸缺失，在推导的氨基酸水平上具有96.42%的同一性。
