Chuang S E, Cheng A L, Lin J K, Kuo M L
Division of Cancer Research, National Health Research Institute, Taipei, Taiwan.
Food Chem Toxicol. 2000 Nov;38(11):991-5. doi: 10.1016/s0278-6915(00)00101-0.
Curcumin (CCM), a major yellow pigment of turmeric obtained from powdered rhizomes of the plant Curcuma longa Linn, is commonly used as coloring agent in foods, drugs and cosmetics. In this study we report that gavage administration of 200 mg/kg or 600 mg/kg CCM effectively suppressed diethylnitrosamine (DEN)-induced liver inflammation and hyperplasia in rats, as evidenced by histopathological examination. Immunoblotting analysis showed that CCM strongly inhibited DEN-mediated the increased expression of oncogenic p21(ras) and p53 proteins in liver tissues of rats. In cell-cycle-related proteins, CCM selectively reduced the expression of proliferating cell nuclear antigen (PCNA), cyclin E and p34(cdc2), but not Cdk2 or cyclin D1. Moreover, CCM also inhibited the DEN-induced increase of transcriptional factor NF-kappa B. However, CCM failed to affect DEN-induced c-Jun and c-Fos expression. It has become widely recognized that the development of human hepatocellular carcinoma (HCC) is predominantly due to the chronic inflammation by virus, bacteria or chemical. Our results suggest a potential role for CCM in the prevention of HCC.
姜黄素（CCM）是从植物姜黄的根茎粉末中提取的主要黄色色素，常用于食品、药品和化妆品的着色剂。在本研究中，我们报告称，经口灌胃给予200mg/kg或600mg/kg的CCM可有效抑制二乙基亚硝胺（DEN）诱导的大鼠肝脏炎症和增生，组织病理学检查证实了这一点。免疫印迹分析表明，CCM强烈抑制DEN介导的大鼠肝脏组织中致癌性p21（ras）和p53蛋白表达的增加。在细胞周期相关蛋白方面，CCM选择性降低增殖细胞核抗原（PCNA）、细胞周期蛋白E和p34（cdc2）的表达，但不影响Cdk2或细胞周期蛋白D1。此外，CCM还抑制DEN诱导的转录因子NF-κB的增加。然而，CCM未能影响DEN诱导的c-Jun和c-Fos表达。人们普遍认识到，人类肝细胞癌（HCC）的发生主要是由于病毒、细菌或化学物质引起的慢性炎症。我们的结果表明CCM在预防HCC方面具有潜在作用。
