Kinetics of photoreactivation.

This paper summerizes experimental work (most of which is published) in which light flashes were used for an analysis of photoenzymatic repair in vivo and in vitro. The method permits determination of the reaction rate constants for the formation, dark dissociation, and repair photolysis of enzyme-substrate complexes under various conditions, and estimation of the number of photoreactivating enzyme molecules present. Investigation of these characteristics is basic for understanding of the overall photoreactivation kinetics observed in biological systems, its dependence on experimental parameters, and possibly its biological significance.