Gao J, Lin Y, Qiu C
Department of Respiratory Disease, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences, Beijing.
Zhonghua Yi Xue Za Zhi. 1998 Aug;78(8):591-4.
To investigate whether susceptibility or resistance to asthma associated with HLA-DRB alleles and analyze the relationship between HLA-DRB genes and clinical phenotype of asthma (TIgE, sIgE, BHR).
Using PCR-SSP(sequence-specific primer polymerase chain reaction), we tested the frequency distribution of HLA-DRB alleles in 50 asthmatic patients and 80 healthy volunteers from Beijing China. All patients had their serum TIgE, IgE antibody specific to house dust mite measured by RAST, bronchial responsiveness assessed by methacholine bronch-provocation (if FEV1% > or = 70%), and broncho-dilation measurement by inhaling salbutamol.
There was significantly increased gene frequency of alleles DR6(13), DR52 in asthmatics compared with normal controls (17% vs 4.3%, P < 0.01; 50% vs 17.5%, P < 0.01), and RR was 7.55 and 4.7 respectively. The frequency of DR2(15), DR51 was lower in asthmatics than in controls (7% vs 18% P < 0.01; 2% vs 33.8% P < 0.01). The percentage of HLA haplotype DR6(13)-DR52 was higher in asthmatics than in healthy volunteers (20% vs 4%, P < 0.01, RR 6.4). 70% of individuals sharing DR6(13) gene and 56% of subjects carrying DR52 gene had elevated serum d1 sIgE antibody (> or = +4). There was no relationship between HLA-DRB alleles and total IgE, BHR.
Alleles DR6(13), DR52 are significantly implicated in their susceptibility to asthma, at least they may be closely associated with this disorder. Conversely DR2(15), DR51 alleles might confer protection against asthma. Positive associations between DR6(13), DR52 and IgE response to d1 allergen are noted. HLA-DRB genes are particularly involved in regulating human atopic immune response.
研究HLA - DRB等位基因与哮喘易感性或抵抗性的关系，并分析HLA - DRB基因与哮喘临床表型（总IgE、特异性IgE、气道高反应性）之间的关系。
采用PCR - SSP（序列特异性引物聚合酶链反应）检测了来自中国北京的50例哮喘患者和80名健康志愿者中HLA - DRB等位基因的频率分布。所有患者均通过RAST检测血清总IgE、针对屋尘螨的IgE抗体，通过乙酰甲胆碱支气管激发试验评估支气管反应性（若第一秒用力呼气容积百分比≥70%），并通过吸入沙丁胺醇进行支气管扩张测量。
与正常对照组相比，哮喘患者中DR6(13)、DR52等位基因的基因频率显著增加（17%对4.3%，P < 0.01；50%对17.5%，P < 0.01），相对危险度分别为7.55和4.7。哮喘患者中DR2(15)、DR51的频率低于对照组（7%对18%，P < 0.01；2%对33.
