Zhao Jing, Gu Ming-lian, Bai Juan, Song Xin, Sun Yu-jing, Qiu Chang-chun
Center for Asthma Research and Education, Capital Institute of Pediatrics, Beijing 100020, China.
Zhonghua Yi Xue Za Zhi. 2009 Aug 25;89(32):2257-60.
To investigate the distribution of HLA-DRB alleles among the asthmatic children and find the alleles with a correlation with susceptibility or resistance to childhood asthma.
The sequence-specific polymerase chain reaction (SSP-PCR) was used to analyze the HLA-DRB genotype in 117 asthmatic children and 120 healthy children in Beijing. Then the frequency of each type, odds ratio (OR) and the 95% confidence interval (CI) of OR were calculated.
The frequency of DR2 (15) in the asthmatic children was 12.0% (28/234) vs 5.4% (13/240) in the healthy children. The frequency was significantly higher than that of nonasthmatic children (OR = 2.590, 95% CI = 1.266-5.298, Chi(2) = 6.431, P < 0.05). Conversely, the frequencies of DR4, DR6 (1402), DR9 and DR53 in asthmatic children [9.4% (22/234), 0.9% (2/234),17.1% (40/234), 29.5% (69/234)] were significantly lower than that of the healthy children [16.3% (39/240), 5.0% (12/240), 31.3% (75/240), 44.2% (106/ 240)], P = 0.026, 0.008, 0.000, 0.001, OR = 0.481, 0.157, 0.312, 0.190. Multi-variate logistic regression demonstrated that the 95% CI of OR for each allele was 1.010-2.245, 0.757-1.116, 0.603-1.054, 0.855-1.014, 0.971-1.010, respectively.
The allele HLA-DR2 (15) is correlated with the susceptibility to childhood asthma whereas it is not related with the severity of asthma.
研究HLA-DRB等位基因在北京地区哮喘儿童中的分布情况，寻找与儿童哮喘易感性或抵抗性相关的等位基因。
采用序列特异性聚合酶链反应（SSP-PCR）技术，对117例哮喘儿童和120例健康儿童的HLA-DRB基因型进行分析。计算各基因型频率、优势比（OR）及OR的95%置信区间（CI）。
哮喘儿童中DR2（15）基因频率为12.0%（28/234），健康儿童为5.4%（13/240），哮喘儿童的该基因频率显著高于非哮喘儿童（OR = 2.590，95%CI = 1.266 - 5.298，χ² = 6.431，P < 0.05）。相反，哮喘儿童中DR4、DR6（1402）、DR9和DR53基因频率[9.4%（22/234）、0.9%（2/234）、17.1%（40/234）、29.5%（69/234）]显著低于健康儿童[16.3%（39/240）、5.0%（12/240）、31.3%（75/240）、44.2%（106/240）]，P = 0.026、0.008、0.000、0.001，OR = 0.481、0.157、0.312、0.190。多因素logistic回归分析显示，各等位基因OR的95%CI分别为1.010 - 2.245、0.757 - 1.116、0.603 - 1.054、0.855 - 1.014、0.971 - 1.010。
HLA-DR2（15）等位基因与儿童哮喘易感性相关，而与哮喘严重程度无关。
