Yang Y, Zhu C, Chen X
Department of Pediatrics, Xiang Ya Hospital, Hunan Medical University, Changsha.
Zhonghua Yi Xue Za Zhi. 1998 Aug;78(8):630-2.
To evaluate the inhibitory effect of baicalin on iron-dependent lipid peroxidation (IDLPO) and the protective effect on pertussis bacilli-induced brain edema in rats.
In vitro, the inhibitory effect of baicalin and deferoxamine (DFX) was studied at different concentrations on IDLPO in brain homogenates of Sprague-Dawley rats. In vivo, the protective effect was studied of baicalin and DFX on brain edema induced by injecting purtussis bacilli into the left internal carotid artery in rats. Forty Sprague-Dawley rats were randomly divided into four groups. Group 1 (NS, n = 10), as control; Group 2 (PB, n = 10), pertussis bacilli control; Group 3 (DFX, n = 10), PB + deferoxamine; and Group 4 (BAI, n = 10), PB + baicalin.
The baicalin was possessed of an inhibitory effect on IDLPO in rat brain homogenate, moreover, the inhibitory potency of baicalin was approximately 20 times higher than DFX treated alone. In vivo, the increase in the content of water(CW) and Evans blue (EB) was attenuated in groups DFX and BAI (CW: 79.8 +/- 0.7)% and (79.5 +/- 0.6)%; (EB: 11 +/- 7 micrograms/g and 12 +/- 5 micrograms/g) as compared with the group PB (CW: 80.4 +/- 0.9%, EB: 24 +/- 10 micrograms/g). The content of iron was significantly lower than in the left hemispheres (injected side) in the group DFX and the group BAI (79 +/- 9 micrograms/g and 77 +/- 10 micrograms/g) than in the left hemispheres in the group PB(132 +/- 28 micrograms.d-1.g), P < 0.05. The activation of CuZn-SOD (L155 +/- 36 U/mg pr, R177 +/- 75 U/mg pr) was decreased in the group BP compared with the group NS(L 201 +/- 49 U/mg pr, R 249 +/- 49 U/mg pr), P < 0.05. The activation of CuZn-SOD was significantly higher in the left brain tissues in the groups DFX and BAI (222 +/- 49 U/mg pr and 230 +/- 45 U/mg pr, respectively) than in the left brain tissues of the group of PB (P < 0.05).
The baicalin and DFX have a protective effects on the pertussis bacilli-induced brain edema in rats, and the protective effects are related to the inhibitory effect on IDLPO, chelating Fe2+, and activated CuZn-SOD.
评价黄芩苷对铁依赖性脂质过氧化（IDLPO）的抑制作用以及对百日咳杆菌诱导的大鼠脑水肿的保护作用。
体外实验中，研究了不同浓度的黄芩苷和去铁胺（DFX）对Sprague-Dawley大鼠脑匀浆中IDLPO的抑制作用。体内实验中，研究了黄芩苷和DFX对大鼠经左颈内动脉注射百日咳杆菌诱导的脑水肿的保护作用。40只Sprague-Dawley大鼠随机分为四组。第1组（NS，n = 10）为对照组；第2组（PB，n = 10）为百日咳杆菌对照组；第3组（DFX，n = 10）为PB + 去铁胺组；第4组（BAI，n = 10）为PB + 黄芩苷组。
黄芩苷对大鼠脑匀浆中的IDLPO具有抑制作用，而且黄芩苷的抑制效力比单独使用DFX高约20倍。体内实验中，与PB组（脑含水量（CW）：80.4 +/- 0.9%，伊文思蓝（EB）：24 +/- 10微克/克）相比，DFX组和BAI组的CW（分别为79.8 +/- 0.7%和79.5 +/- 0.6%）以及EB（分别为11 +/- 7微克/克和12 +/- 5微克/克）的增加有所减轻。DFX组和BAI组的铁含量（分别为79 +/- 9微克/克和77 +/- 10微克/克）显著低于PB组左半球（注射侧）的铁含量（132 +/- 28微克·天⁻¹·克），P < 0.05。与NS组（左侧：201 +/- 49 U/mg蛋白，右侧：249 +/- 49 U/mg蛋白）相比，BP组的铜锌超氧化物歧化酶（CuZn-SOD）活性（左侧：155 +/- 36 U/mg蛋白，右侧：177 +/- 75 U/mg蛋白）降低，P < 0.05。DFX组和BAI组左脑组 织中的CuZn-SOD活性（分别为222 +/- 49 U/mg蛋白和230 +/- 45 U/mg蛋白）显著高于PB组左脑组 织（P < 0.05）。
黄芩苷和DFX对百日咳杆菌诱导的大鼠脑水肿具有保护作用，且保护作用与对IDLPO的抑制作用、螯合Fe²⁺以及激活CuZn-SOD有关。
