Chen X, Yang Y, Tao Y
Department of Pediatrics, Xiangya Hospital, Hunan Medical University, Changsha.
Hunan Yi Ke Da Xue Xue Bao. 1997;22(4):301-3.
To induced new model of delayed infection brain injury, we divided randomly 40 adult male Sprague-Dawley rats into four groups: bordetella pertussis (BP) groups, and normal sailine (NS) groups in 4 h (n = 10) and 24 h (n = 10), respectively, injected BP at 0.2 ml.kg-1 which contained the bacilli in 10.8 x 10(9).ml-1 into the rat left internal carotid artery of BP groups, and injected at the equal volume as control in the same way into NS groups. The water content (WC), Evans blue content (EB), cations of sodium, potassium, calcium in the brain tissues were measured and the morphologic changes in the hippocampal CA1 were detected by electron microscopy. These results were shown as follows: the left hemisphere of BP group in 4 h was characterized by the brain edema which predominated in swollen, necrotic astroglial cells, endothelial cells, and insignificant increase of contents of sodium, calcium and decrease of potassium; BP group in 24 h was characterized by dark cells, the brain edema also existed in astroglial cells. These results indicate that a delayed infection brain injury model is performed successfully by injection with BP into the left internal carotid artery of rats. The model is characterized by the features of delayed neuronal death and mixed type brain edema.
为诱导新型迟发性感染性脑损伤模型，我们将40只成年雄性Sprague-Dawley大鼠随机分为四组：百日咳博德特氏菌（BP）组以及4小时（n = 10）和24小时（n = 10）的生理盐水（NS）组，分别向BP组大鼠的左颈内动脉注射0.2 ml.kg-1含10.8 x 10(9).ml-1杆菌的BP，以同样方式向NS组注射等量作为对照的液体。测量脑组织中的含水量（WC）、伊文思蓝含量（EB）、钠、钾、钙阳离子，并通过电子显微镜检测海马CA1区的形态学变化。结果如下：4小时BP组左半球以脑水肿为特征，主要表现为肿胀、坏死的星形胶质细胞、内皮细胞，钠、钙含量显著增加，钾含量降低；24小时BP组以细胞变黑为特征，星形胶质细胞中也存在脑水肿。这些结果表明，通过向大鼠左颈内动脉注射BP成功建立了迟发性感染性脑损伤模型。该模型的特征为迟发性神经元死亡和混合型脑水肿。
