[7种药物对牛睾丸醛糖还原酶的抑制作用]

[Inhibitory effect of 7 agents on bovine testis aldose reductase].

作者信息

Liu C, Zhu X

机构信息

Huashan Hospital of Shanghai Medical University.

出版信息

Zhongguo Zhong Yao Za Zhi. 1997 Jun;22(6):372-3, inside back cover.

PMID:11038893

Abstract

The inhibitory effects of seven agents on aldose reductase were studied by enzymatic kinetics using the purified enzyme from bovine testis, sorbinil, tolrestat, silybin and berberini were found to exhibit a marked enzyme-inhibitory effect with Ki values of 1.08 mumol/L, 0.12 mumol/L, 3.5 micrograms/ml, and 2.7 micrograms/ml respectively. The established assay system described in this paper may be useful in screening aldose reductase inhibitors.

摘要

采用从牛睾丸中纯化得到的醛糖还原酶，通过酶动力学研究了七种药物的抑制作用。发现索比尼尔、托瑞司他、水飞蓟宾和小檗碱对该酶具有显著的抑制作用，其抑制常数（Ki）值分别为1.08 μmol/L、0.12 μmol/L、3.5 μg/ml和2.7 μg/ml。本文所描述的已建立的检测系统可能有助于筛选醛糖还原酶抑制剂。