Li C, Gui P, He X, Yang H
Beijing General Hospital of PLA.
J Tradit Chin Med. 2000 Sep;20(3):216-22.
This study is to explore the actions of nitric oxide (NO) and inducible nitric oxide synthase (iNOS) on endotoxin (lipopolysaccharide, LPS) induced rat acute lung injury (ALI) and effect of Rhubarb on them. LPS was injected into the sublingual vein of male Wistar rats to prepare ALI animal models. The rats were divided into 4 groups: LPS, control, Rhubarb, and dexamethasone. Macroscopic and histopathological examinations of the lung specimens were performed and the biological indexes of lung, including wet weight/dry weight, the rate of neutrophils and protein content in the pulmonary alveolar lavage fluid, pulmonary vascular permeability and pulmonary alveolar permeability were observed. In the mean time, the contents of serum NO and the activities of lung tissue homogenate iNOS were measured. The results showed that in the LPS group, the injury and celluar infiltration in the pulmonary stroma and alveoli were more prominent than that in the control group. Lung wet weight/dry weight, the rate of neutrophils, protein content, pulmonary alveolar permeability, pulmonary vascular permeability were significantly increased (P < 0.01); NO and iNOS were also markedly elevated (P < 0.01). In the groups of dexamethasone and Rhubarb, the histopathological changes were significantly milder, and all the above biological indexes of lung injury and the contents of NO and the activities of iNOS were correspondingly decreased (P < 0.05). The above data demonstrate that NO and iNOS play an important role in the onset of ALI; dexamethasone and Rhubarb interfering treatment can ameliorate lung injury and decrease the concentrations of NO and the activities of iNOS, showing that through inhibiting the levels of NO and the activities of iNOS, these 2 agents exert protective effect on ALI induced LPS.
本研究旨在探讨一氧化氮（NO）及诱导型一氧化氮合酶（iNOS）在内毒素（脂多糖，LPS）诱导的大鼠急性肺损伤（ALI）中的作用以及大黄对其的影响。将LPS注入雄性Wistar大鼠舌下静脉制备ALI动物模型。大鼠分为4组：LPS组、对照组、大黄组和地塞米松组。对肺标本进行大体和组织病理学检查，并观察肺的生物学指标，包括湿重/干重、肺泡灌洗液中中性粒细胞比例和蛋白含量、肺血管通透性和肺泡通透性。同时，检测血清NO含量及肺组织匀浆iNOS活性。结果显示，LPS组肺间质和肺泡的损伤及细胞浸润较对照组更明显。肺湿重/干重、中性粒细胞比例、蛋白含量、肺泡通透性、肺血管通透性显著升高（P<0.01）；NO和iNOS也明显升高（P<0.01）。地塞米松组和大黄组组织病理学改变明显较轻，上述所有肺损伤生物学指标及NO含量和iNOS活性相应降低（P<0.05）。上述数据表明，NO和iNOS在ALI发病中起重要作用；地塞米松和大黄干预治疗可减轻肺损伤，降低NO浓度和iNOS活性，表明这两种药物通过抑制NO水平和iNOS活性，对LPS诱导的ALI发挥保护作用。
