Chunsheng L, Peichun G, Xinhua H
General Hospital of Beijing Command, Beijing 100700.
Chin Med Sci J. 2000 Jun;15(2):93-7.
To approach the relation and the possible mechanism between the expression of intercellular adhesion molecule (ICAM-1) mRNA and acute lung injury (ALI) and the mechanisms of rhubarb in the prevention and treatment of the lung injury.
Lipopolysaccharide (LPS) was injected into the sublingual vein of male Wistar rats to perform ALI animal model. The rats were divided into 4 groups: LFS group, control group, rhubarb group and dexamethasone group. Macroscopic and histopathological examinations were performed and biological markers were measured for the lung specimens. The markers included lung wet/dry weight, the rate of neutrophils and protein content in the pulmonary alveolar lavage fluid, pulmonary vascular permeability and pulmonary alveolar permeability index. Molecular hybridization method was used to determine the expression of ICAM-1 mRNA.
In the lung tissues, the ICAM-1 mRNA expression was increased in the endothelial cells of pulmonary veins and capillaries, rhubarb and dexamethasone had the action of decreasing the expression. The light reflex value in the gray scale scanning showed that in the comparison between the LPS and the control group, the gray scale value of the lung tissues in ALI was significantly increased, thus the light reflex value was markedly decreased (P < 0.01), demonstrating the expression of ICAM-1 mRNA was increased. In comparison with the LPS group, dexamethasone and rhubarb could decrease the gray scale value of the lung tissue significantly, thus the light reflex value was elevated (P < 0.01, P< 0.05); the corresponding pathologic change of lung tissue and the biological markers of the lung injury were significantly decreased or ameliorated.
The increase of the expression of ICAM-1 mRNA in the lung tissues of ALI plays the roles in ALI. The application of rhubarb and dexamethasone can decease the expression and ameliorate the lung damage; its mechanism is possibly via the inhibition of ICAM-1 mRNA expression.
探讨细胞间黏附分子（ICAM-1）mRNA表达与急性肺损伤（ALI）的关系及可能机制，以及大黄防治肺损伤的机制。
将脂多糖（LPS）注入雄性Wistar大鼠舌下静脉制备ALI动物模型。大鼠分为4组：LPS组、对照组、大黄组和地塞米松组。对肺标本进行大体和组织病理学检查，并检测生物学指标。指标包括肺湿/干重、肺泡灌洗液中中性粒细胞比例和蛋白含量、肺血管通透性和肺泡通透指数。采用分子杂交法检测ICAM-1 mRNA的表达。
肺组织中，肺静脉和毛细血管内皮细胞ICAM-1 mRNA表达增加，大黄和地塞米松具有降低其表达的作用。灰度扫描光反射值显示，LPS组与对照组比较，ALI肺组织灰度值显著升高，光反射值明显降低（P<0.01），表明ICAM-1 mRNA表达增加。与LPS组比较，地塞米松和大黄能显著降低肺组织灰度值，光反射值升高（P<0.01，P<0.05）；肺组织相应病理改变及肺损伤生物学指标明显降低或改善。
ALI肺组织中ICAM-1 mRNA表达增加在ALI中起作用。大黄和地塞米松的应用可降低其表达并改善肺损伤；其机制可能是通过抑制ICAM-1 mRNA表达。
