Fridell R A, Lussi A, Crenshaw M A, Bawden J W
Dental Research Center, University of North Carolina, Chapel Hill 27599, USA.
J Dent Res. 1988 Feb;67(2):487-90. doi: 10.1177/00220345880670021101.
The objectives of this study were to determine the specific surface area of secretory-stage and of maturation-stage enamel, to compare the fluoride uptake by isolated enamel at these two stages on a surface-area basis, and to examine the effect of the organic matrix on the fluoride uptake by whole enamel. Fetal bovine secretory and maturation stage enamel samples were collected, and a portion of the enamel at each developmental stage was treated with hydrazine for removal of the organic matrix. The specific surface areas of the enamel mineral, as determined by the multi-point BET method, were 59.3 m2/g in the secretory stage and 37.9 m2/g in the maturation stage. Whole and deproteinated enamel samples were equilibrated in buffered solutions containing 10(-5) to 10(-3) mol/L fluoride, and the uptake was measured with a fluoride specific electrode. The results indicate that the in vitro fluoride uptake was controlled solely by the surface area of the apatitic mineral and that the organic matrix did not contribute to the fluoride uptake.
本研究的目的是测定分泌期和成熟阶段牙釉质的比表面积，在表面积基础上比较这两个阶段分离牙釉质对氟的摄取，并研究有机基质对全牙釉质氟摄取的影响。收集胎牛分泌期和成熟阶段的牙釉质样本，每个发育阶段的一部分牙釉质用肼处理以去除有机基质。通过多点BET法测定，牙釉质矿物质的比表面积在分泌期为59.3平方米/克，在成熟阶段为37.9平方米/克。将完整的和脱蛋白的牙釉质样本在含有10^(-5)至10^(-3)摩尔/升氟的缓冲溶液中平衡，并用氟特异性电极测量摄取量。结果表明，体外氟摄取仅受磷灰石矿物质表面积的控制，有机基质对氟摄取没有贡献。
