Wu P, Sato K, Suzuta F, Hikasa Y, Kagota K
Department of Veterinary Internal Medicine, Faculty of Agriculture, Tottori University, Koyama, Japan.
J Vet Med Sci. 2000 Sep;62(9):933-9. doi: 10.1292/jvms.62.933.
The effects of several factors related to lipids on bovine adipocyte differentiation were investigated in primary culture. Adipocyte differentiation was assessed by development of glycerol-3-phosphate dehydrogenase (GPDH) activity and morphological observation. Addition of triglyceride mixture (Intralipid), caprylic acid and very low-, low- and high-density lipoproteins (VLDL, LDL and HDL) stimulated bovine preadipocyte differentiation in serum-free condition. Especially, VLDL strongly increased both cell protein contents and GPDH activity, suggesting that it stimulated both proliferation and differentiation of bovine preadipocytes. Under Intralipid-induced condition, differentiation of preadipocytes from subcutaneous adipose tissues was more evident than those from omental adipose tissues. However, such depot difference was not observed in medium supplemented with indomethacin, which is a peroxisome proliferator-activated receptor (PPAR) gamma agonist. This suggests that the differentiation capacity of bovine preadipocytes was different between depots and such difference is dependent on the ability to utilize lipids as endogenous PPARgamma ligands. Therefore, lipid metabolites have the stimulatory effects on bovine adipocyte differentiation in vitro, and lipoproteins, especially VLDL, may play an important role in development of bovine adipose tissues in vivo.
在原代培养中研究了几种与脂质相关的因素对牛脂肪细胞分化的影响。通过甘油-3-磷酸脱氢酶（GPDH）活性的发展和形态学观察来评估脂肪细胞的分化。添加甘油三酯混合物（英脱利匹特）、辛酸以及极低密度脂蛋白、低密度脂蛋白和高密度脂蛋白（VLDL、LDL和HDL）可在无血清条件下刺激牛前脂肪细胞的分化。特别是，VLDL强烈增加细胞蛋白含量和GPDH活性，表明它刺激了牛前脂肪细胞的增殖和分化。在英脱利匹特诱导的条件下，皮下脂肪组织前脂肪细胞的分化比网膜脂肪组织的更明显。然而，在添加了吲哚美辛（一种过氧化物酶体增殖物激活受体（PPAR）γ激动剂）的培养基中未观察到这种部位差异。这表明牛前脂肪细胞的分化能力在不同部位之间存在差异，且这种差异取决于将脂质用作内源性PPARγ配体的能力。因此，脂质代谢产物在体外对牛脂肪细胞分化具有刺激作用，脂蛋白，尤其是VLDL，可能在体内牛脂肪组织的发育中起重要作用。
