Park S, Ohya F, Yamashita K, Nishifuji K, Iwasaki T
Veterinary Medical Teaching Hospital, Gifu University, Japan.
J Vet Med Sci. 2000 Sep;62(9):983-8. doi: 10.1292/jvms.62.983.
The intradermal skin test (IDST) and serologic allergy test (SAT) has been developed for confirming a diagnosis of canine atopy and determining allergens for immunotherapy. To determine the prevalence of causative allergens for canine atopic dermatitis in Japan, IDST and SAT were performed with the CMG Immunodot strips on 95 atopic dogs using 9 allergens. In addition, we compared agreement rate, sensitivity and specificity between them (using IDST as the standard). The allergen most commonly positive in both tests was house dust mites (IDST: 69.5%, SAT: 48.4%). Moreover, Japanese cedar, mugwort and grass mix were detected as attendant causative allergens. Agreement rates between the two tests ranged from 67.4% to 96.8%; the overall mean agreement rate were 81%. SAT was shown to have sensitivity to IDST ranging from 16.7 to 68.2%. The specificities were very high for all allergens, on the order of 94.9-100% (median=98.7%). Finally, the efficacy of immunotherapy was evaluated on 27 atopic dogs based on IDST (15 dogs) and SAT (12 dogs) results. Overall, 60% (9/15) of the IDST group and 66.8% (8/12) of the SAT group experienced a 50% to 100% reduction in their symptomatology. No significant differences were found in response to immunotherapy during the follow-up period between allergen selection methods. These results indicate the value of serologic tests as an aid to identifying an allergen solution for immunotherapy.
皮内皮肤试验（IDST）和血清学过敏试验（SAT）已被开发用于确诊犬特应性皮炎并确定免疫疗法的过敏原。为了确定日本犬特应性皮炎致病过敏原的流行情况，使用9种过敏原对95只特应性犬进行了IDST和CMG免疫斑点试纸条SAT检测。此外，我们比较了两者之间的符合率、敏感性和特异性（以IDST为标准）。两种检测中最常见的阳性过敏原是屋尘螨（IDST：69.5%，SAT：48.4%）。此外，日本柳杉、艾蒿和混合草被检测为伴随致病过敏原。两种检测之间的符合率在67.4%至96.8%之间；总体平均符合率为81%。SAT对IDST的敏感性范围为16.7%至68.2%。所有过敏原的特异性都非常高，在94.9 - 100%之间（中位数 = 98.7%）。最后，根据IDST（15只犬）和SAT（12只犬）的结果对27只特应性犬的免疫疗法疗效进行了评估。总体而言，IDST组60%（9/15）和SAT组66.8%（8/12）的症状减轻了50%至100%。在随访期间，过敏原选择方法之间对免疫疗法的反应未发现显著差异。这些结果表明血清学检测在辅助确定免疫疗法过敏原解决方案方面的价值。
