家禽源和人源葡萄球菌分离株中红霉素抗性决定因素的比较分子分析
Comparative molecular analysis of erythromycin-resistance determinants in staphylococcal isolates of poultry and human origin.
作者信息
Nawaz M S, Khan S A, Khan A A, Khambaty F M, Cerniglia C E
机构信息
Division of Microbiology, National Center for Toxicological Research/FDA, 3900 NCTR Road, Jefferson, AR 72079, USA.
出版信息
Mol Cell Probes. 2000 Oct;14(5):311-9. doi: 10.1006/mcpr.2000.0320.
The ermA, ermB, ermC and msrA/msrB genes were detected in multidrug-resistant Staphylococcus spp. strains by PCR. Among 25 human clinical staphylococcal isolates the ermA, ermB, ermC and the msrA/msrB genes were detected in 88, 72, 4 and 100% of the strains, respectively. Among 24 poultry isolates the ermA, ermB, ermC and the msrA/msrB genes were detected in 100, 16.6, 50 and 12.5% of the strains, respectively. The ermA gene was found exclusively on the chromosome, whereas the ermC gene was found on 2.4-4.2 kb plasmids. Restriction fragment length polymorphism (RFLP) analysis of the ermA gene with Eco RI revealed five patterns (25.0, 21.0, 10.5, 6.2 and 4. 8 kb) for the clinical strains and two (8.0 and 6.2 kb) for the poultry strains. The 6.2 kb RFLP pattern, in both the poultry and human clinical isolates, indicates a common lineage for the ermA gene.
通过聚合酶链反应（PCR）在多重耐药葡萄球菌属菌株中检测到ermA、ermB、ermC和msrA/msrB基因。在25株人类临床葡萄球菌分离株中，分别在88%、72%、4%和100%的菌株中检测到ermA、ermB、ermC和msrA/msrB基因。在24株家禽分离株中，分别在100%、16.6%、50%和12.5%的菌株中检测到ermA、ermB、ermC和msrA/msrB基因。ermA基因仅存在于染色体上，而ermC基因存在于2.4 - 4.2 kb的质粒上。用Eco RI对ermA基因进行限制性片段长度多态性（RFLP）分析，临床菌株显示出五种模式（25.0、21.0、10.5、6.2和4.8 kb），家禽菌株显示出两种模式（8.0和6.2 kb）。在家禽和人类临床分离株中均出现的6.2 kb RFLP模式表明ermA基因存在共同的谱系。
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