Balogh S A, Kwon Y T, Denenberg V H
Biobehavioral Sciences Graduate Degree Program, University of Connecticut, Storrs, Connecticut 06269, USA.
Learn Mem. 2000 Sep-Oct;7(5):279-86. doi: 10.1101/lm.33500.
The N-end rule is one ubiquitin-proteolytic pathway that relates the in vivo half-life of a protein to the identity of its N-terminal residue. NTAN1 deamidates N-terminal asparagine to aspartate, which is conjugated to arginine by ATE1. An N-terminal arginine-bearing substrate protein is recognized, ubiquitylated by UBR1/E3alpha, and subsequently degraded by 26S proteasomes. Previous research showed that NTAN1-deficient mice exhibited impaired long-term memory in the Lashley III maze. Therefore, a series of studies, designed to assess the role of NTAN1 in short- and intermediate-term memory processes, was undertaken. Two hundred sixty mice (126 -/-; 134 +/ +) received Lashley III maze training with intertrial intervals ranging from 2-180 min. Results indicated that inactivation of NTAN1 amidase differentially affects short-, intermediate-, and long-term memory.
N端规则是一种泛素-蛋白水解途径，它将蛋白质在体内的半衰期与其N端残基的身份联系起来。NTAN1将N端天冬酰胺脱酰胺化为天冬氨酸，后者由ATE1与精氨酸缀合。携带N端精氨酸的底物蛋白被识别，由UBR1/E3α进行泛素化，随后被26S蛋白酶体降解。先前的研究表明，NTAN1缺陷小鼠在拉什利三世迷宫中表现出长期记忆受损。因此，开展了一系列旨在评估NTAN1在短期和中期记忆过程中作用的研究。260只小鼠（126只基因敲除小鼠；134只野生型小鼠）接受了拉什利三世迷宫训练，试验间隔时间为2至180分钟。结果表明，NTAN1酰胺酶的失活对短期、中期和长期记忆有不同影响。
