Pintureau B, Chaudier S, Lassablière F, Charles H, Grenier S
Biologie Fonctionnelle, Insectes et Interactions-UMR INRA/INSA de Lyon, INSA Bâtiment 406, 69 621 Villeurbanne cedex, France.
J Mol Evol. 2000 Oct;51(4):374-7. doi: 10.1007/s002390010099.
Wolbachia are symbiotic bacteria altering reproductive characters of numerous arthropods. Their most recent phylogeny and classification are based on sequences of the wsp gene. We sequenced wsp gene from six Wolbachia strains infecting six Trichogramma species that live as egg parasitoids on many insects. This allows us to test the effect of the addition of sequences on the Wolbachia phylogeny and to check the classification of Wolbachia infecting Trichogramma. The six Wolbachia studied are classified in the B supergroup. They confirm the monophyletic structure of the B Wolbachia in Trichogramma but introduce small differences in the Wolbachia classification. Modifications include the definition of a new group, Sem, for Wolbachia of T. semblidis and the merging of the two closely related groups, Sib and Kay. Specific primers were determined and tested for the Sem group.
沃尔巴克氏体是共生细菌，可改变众多节肢动物的生殖特征。它们最新的系统发育和分类基于wsp基因序列。我们对感染六种赤眼蜂的六个沃尔巴克氏体菌株的wsp基因进行了测序，这些赤眼蜂以许多昆虫的卵为寄生对象。这使我们能够测试添加序列对沃尔巴克氏体系统发育的影响，并检验感染赤眼蜂的沃尔巴克氏体的分类。所研究的六个沃尔巴克氏体菌株被归类于B超群。它们证实了赤眼蜂中B组沃尔巴克氏体的单系结构，但在沃尔巴克氏体分类中引入了一些小差异。修改内容包括为拟澳洲赤眼蜂的沃尔巴克氏体定义一个新组Sem，以及将两个密切相关的组Sib和Kay合并。确定并测试了Sem组的特异性引物。
