Ono M, Braig H R, Munstermann L E, Ferro C, O'Neill S L
Department of Epidemiology and Public Health, Yale University School of Medicine, New Haven, CT 06520, USA.
J Med Entomol. 2001 Mar;38(2):237-41. doi: 10.1603/0022-2585-38.2.237.
Old and New World phlebotomine sand fly species were screened for infection with Wolbachia, intracellular bacterial endosymbionts found in many arthropods and filarial nematodes. Of 53 samples representing 15 species, nine samples offour species were found positive for Wolbachia by polymerase chain reaction amplification using primers for the Wolbachia surface protein (wsp) gene. Five of the wsp gene fragments from four species were cloned, sequenced, and used for phylogenetic analysis. These wsp sequences were placed in three different clades within the arthropod associated Wolbachia (groups A and B), suggesting that Wolbacia has infected sand flies on more than one occasion. Two distantly related sand fly species, Lutzomyia (Psanthyromyia) shannoi (Dyar) and Lutzomyia (Nyssomyia) whitmani (Antunes & Coutinho), infected with an identical Wolbachia strain suggest a very recent horizontal transmission.
对新旧世界的白蛉物种进行了沃尔巴克氏体感染筛查，沃尔巴克氏体是在许多节肢动物和丝虫线虫中发现的细胞内细菌内共生体。在代表15个物种的53个样本中，通过使用针对沃尔巴克氏体表面蛋白（wsp）基因的引物进行聚合酶链反应扩增，发现来自4个物种的9个样本的沃尔巴克氏体呈阳性。对来自4个物种的5个wsp基因片段进行了克隆、测序，并用于系统发育分析。这些wsp序列被置于节肢动物相关沃尔巴克氏体（A组和B组）内的三个不同进化枝中，这表明沃尔巴克氏体不止一次感染白蛉。两种亲缘关系较远的白蛉物种，即感染了相同沃尔巴克氏体菌株的沙氏白蛉（Lutzomyia (Psanthyromyia) shannoi (Dyar)）和惠特曼白蛉（Lutzomyia (Nyssomyia) whitmani (Antunes & Coutinho)），表明最近发生了水平传播。
