Anderson D E, Kim J H, Hancock J D, Han I K
Department of Veterinary Clinical Sciences, College of Veterinary Medicine, Ohio State University, Columbus 43210, USA.
Contemp Top Lab Anim Sci. 2000 Sep;39(5):26-8.
The purpose of our study was to develop a surgical method for collection of ileal digesta in neonatal (< 5 kg) pigs and to determine potential complications of the procedure. In 18 10-day-old pigs, we performed ileocutaneous anastomosis (ICA) via a right ventrolateral incision. The ICA was readily performed in these neonatal pigs; one pig died 24 hours after surgery because of intestinal volvulus. Pigs were monitored twice daily for development of post-operative complications. Ileal digesta were collected "free-catch" by using metabolism cages because attempts to use cannulas (diameter, 4 to 8 mm) and collection bags failed. To determine the effect of colon bypass on hydration, electrolytes, glucose, and serum enzyme activities, we collected serum biochemistry data before and 6 days after surgery. Changes in serum biochemical values included increased potassium, creatinine, total protein, albumin, and globulin and decreased ALP and glucose, but all values remained within normal ranges for neonatal pigs. ICA is tolerated well by neonatal pigs and is an easily learned and rapid technique for collection of ileal digesta. In addition, ICA is a useful alternative to "T-cannulas" and ileorectal anastomosis for nutrition research using neonatal pigs weighing < 5 kg.
我们研究的目的是开发一种用于收集新生仔猪（体重<5 kg）回肠食糜的手术方法，并确定该手术可能出现的并发症。在18头10日龄的仔猪中，我们通过右侧腹外侧切口进行了回肠皮肤吻合术（ICA）。在这些新生仔猪中，ICA操作很容易完成；有一头仔猪在术后24小时因肠扭转死亡。每天对仔猪进行两次监测，观察术后并发症的发生情况。由于使用插管（直径4至8毫米）和收集袋的尝试均告失败，因此使用代谢笼“自由采集”回肠食糜。为了确定结肠旁路对水合作用、电解质、葡萄糖和血清酶活性的影响，我们在手术前和术后6天收集了血清生化数据。血清生化值的变化包括钾、肌酐、总蛋白、白蛋白和球蛋白升高，碱性磷酸酶和葡萄糖降低，但所有值均保持在新生仔猪的正常范围内。新生仔猪对ICA耐受性良好，这是一种易于掌握且快速收集回肠食糜的技术。此外，对于体重<5 kg的新生仔猪的营养研究，ICA是“T形插管”和回直肠吻合术的一种有用替代方法。
