Hennig U, Noel R, Herrmann U, Wünsche J, Mehnert E
Arch Tierernahr. 1986 Jul;36(7):585-96. doi: 10.1080/17450398609425299.
For the purpose of studying nutrient digestion and absorption in the small intestine, pigs were provided with ileo-rectal anastomoses. The anaesthetic and operation method is described. X-ray examinations do not show a significant backflow of digesta into the from colon cranially open towards the anastomosis as well as a totally intact sphincter ani. With the help of additional doses of highly digestible, N-free nutrients rich in energy (starch + saccharose), the operated animals achieved a growth development corresponding to that of intact control animals. For the assessment of the physiologic state of the animals, the concentrations in the serum of the total protein, of albumin, urea, glucose, calcium, inorganic phosphorus and creatinine as well as the activities of aspartate amino transferase and alkaline phosphatase were determined. These showed for the respective criteria changes conditioned by age and dependent on feed intake. The fixing of the anastomoses did not result in disturbances of nutritional or organic damages. Histologic studies of the mucosa of stomach, duodenum and jejunum did not show pathologic changes. The weight of stomach, duodenum and jejunum, determined in 2 live weight ranges, is identical for operated and intact animals in relation to the weight of the empty body of the animals. The weight of ileum, caecum and colon of the operated animals is significantly lower, due to inactivity atrophy. The tissue amount available for the intake of a volume unit (weight-volume index) is nearly identical for operated and intact animals in the duodenum and the jejunum. The index functionally increasing for intact animals from the jejunum to the ileum (stenosis due to temporary digesta congestion in the ileum) remained the same with anastomosis animals for 29 or 26 weeks resp. after the operation. As a result of the biochemical and morphological studies it was shown that animals with an ileo-rectal anastomosis are suitable for the determination of nutrient digestibility at the end of the ileum.
为了研究小肠中的营养物质消化与吸收，给猪进行了回肠 - 直肠吻合术。描述了麻醉和手术方法。X射线检查未显示消化物向头侧经吻合口显著反流至结肠，以及肛门括约肌完全完整。借助额外剂量的高消化率、无氮且富含能量的营养物质（淀粉 + 蔗糖），接受手术的动物实现了与完整对照动物相当的生长发育。为评估动物的生理状态，测定了血清中总蛋白、白蛋白、尿素、葡萄糖、钙、无机磷和肌酐的浓度以及天冬氨酸氨基转移酶和碱性磷酸酶的活性。这些指标显示出因年龄和采食量而产生的各自标准变化。吻合术的固定并未导致营养紊乱或器质性损伤。对胃、十二指肠和空肠黏膜的组织学研究未显示病理变化。在两个体重范围内测定的胃、十二指肠和空肠重量，手术动物和完整动物相对于动物空腹体重是相同的。由于失用性萎缩，手术动物的回肠、盲肠和结肠重量显著更低。在十二指肠和空肠中，手术动物和完整动物每单位体积可利用的组织量（重量 - 体积指数）几乎相同。完整动物从空肠到回肠功能上增加的指数（由于回肠中暂时的消化物淤滞导致狭窄），吻合术动物在术后分别保持29周或26周不变。生化和形态学研究结果表明，进行回肠 - 直肠吻合术的动物适用于测定回肠末端的营养物质消化率。
