Zakharov N L, Lukashev E P, Noks P P, Seĭfullina N Kh, Churbanova I Iu
Biological Department, Lomonosov Moscow State University, Russia.
Biofizika. 2000 Jul-Aug;45(4):648-53.
It is shown that the addition of dipyridamole (2,6-bis(diethanolamino)-4,8-dipiperidinopyrimido[5,4d]py rim idine) (up to 10(-4) M) leads to a drastic acceleration of the dark recombination reaction between photooxidized bacteriochlorophyll and photoreduced primary quinone in reaction centers of Rhodobacter sphaeroides. The value of the acceleration is similar to that registered under cryogenic temperatures. The extent of the effect of dipyridamole derivatives depended on their structure. In wild-type bacteriorhodopsin and D96N mutant, dipyridamole slowed down the Schiff base reprotonation (the kinetics of M412 form decay was registered). It is suggested that dipyridamole can influence the structural and dynamic state of membrane proteins by affecting the system of their hydrogen-bonds and thus modify electron and proton transport processes.
结果表明，加入双嘧达莫（2,6 - 双（二乙醇氨基）- 4,8 - 二哌啶基嘧啶并[5,4 - d]嘧啶）（浓度高达10^(-4) M）会导致球形红杆菌反应中心中光氧化细菌叶绿素与光还原初级醌之间的暗重组反应急剧加速。加速值与在低温下记录的值相似。双嘧达莫衍生物的作用程度取决于其结构。在野生型细菌视紫红质和D96N突变体中，双嘧达莫减缓了席夫碱的再质子化（记录了M412形式衰减的动力学）。有人认为，双嘧达莫可以通过影响膜蛋白的氢键系统来影响其结构和动态状态，从而改变电子和质子传输过程。
