Knox P P, Lukashev E P, Borissevitch G P, Zakharova N I
Biological Department, Lomonosov Moscow State University, Russia.
Membr Cell Biol. 2000;14(2):173-80.
The effect of Dipyridamole (10(-6)-10(-3) M) on the photomobilized electron transport in the system of quinone acceptors Q(A)-Q(B) of isolated photosynthetic reaction centers of Rhodobacter sphaeroides and on its temporary stabilization on Q(B) was studied. Depending on the type of the detergent present in the reaction center (lauryl dimethylamine oxide, Triton X-100, sodium dodecyl sulfate, and sodium cholate), dipyridamole could increase the time of the electron transfer to Q(B). The dipyridamole effect on the efficiency of the electron stabilization on Q(B) for reaction centers with different detergents was revealed in slowing down the process of dark reduction of photoactive bacteriochlorophyll from Q(B) at initial concentrations of added dipyridamole (10(-6)-10(-5) M) with following acceleration of the process at the dipyridamole concentrations of 10(-4)-10(-3) M. The pH lowering from 6.8-7.0 to 5.9-6.0 increased the dipyridamole effect. The possibility of the dipyridamole effect on the structural-dynamic state of the reaction center complex, including its hydrogen bond system, which influences the studied parameters of functional activity, is suggested.
研究了双嘧达莫（10⁻⁶ - 10⁻³M）对球形红细菌分离的光合反应中心醌受体Q(A)-Q(B)系统中光驱动电子传递及其在Q(B)上的暂时稳定作用的影响。根据反应中心中存在的去污剂类型（月桂基二甲基氧化胺、Triton X-100、十二烷基硫酸钠和胆酸钠），双嘧达莫可增加电子转移至Q(B)的时间。在添加双嘧达莫的初始浓度（10⁻⁶ - 10⁻⁵M）下，双嘧达莫对不同去污剂反应中心中Q(B)上电子稳定效率的影响表现为减缓光活性细菌叶绿素从Q(B)的暗还原过程，而在双嘧达莫浓度为10⁻⁴ - 10⁻³M时该过程加速。pH从6.8 - 7.0降至5.9 - 6.0会增强双嘧达莫的作用效果。有人提出双嘧达莫可能对反应中心复合物的结构 - 动态状态产生影响，包括其氢键系统，而这会影响所研究的功能活性参数。
