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[种子紫外线照射后小麦芽抗氧化系统反应的变化]

[Change in the reaction of the antioxidant system of wheat sprouts after UV-irradiation of seeds].

作者信息

Rogozhin V V, Kuriliuk T T, Filippova N P

机构信息

Yakutsk State Agricultural Academy, Russia.

出版信息

Biofizika. 2000 Jul-Aug;45(4):730-6.

Abstract

The response of the antioxidant system of sprouts of wheat Triticum aestivum L. to preliminary irradiation of seeds with UV light was studied. The dependence of lipid peroxidation and the extent of antioxidant activity on the duration of irradiation was studied. It was shown that low doses of UV radiation (5-15 min) stimulate the antioxidant protection of green wheat sprouts grown for eight days. Increasing the irradiation time to 30-60 min leads to the inhibition of lipid peroxidation by the antioxidant system. A more prolonged irradiation of seeds with UV light (for 1-6 h) led to an increase in the level of lipid peroxidation in sprouts. However, 1-2-day-old sprouts from seeds irradiated for 5-6 h, adapted themselves to the influence due to the compensatory mechanisms. By the 8th day of germination of preliminarily irradiated seeds, the content of antioxidants and malone dialdehyde returned to the norm. The dynamics of activity of peroxidase in seeds irradiated with low doses of UV light for 30 min was studied. It was found that on the third day of seed germination, a decrease in peroxidase activity followed by its slight increase occurred. The maximum activity of the enzyme in the endosperm was observed on day 5-6, and in roots and green sprouts, on day 3-5 of germination. It was concluded that antioxidants and peroxidase are involved in the compensatory mechanisms of inhibition of free radicals formed upon UV irradiation of seeds.

摘要

研究了普通小麦(Triticum aestivum L.)种子经紫外线预照射后芽苗抗氧化系统的反应。研究了脂质过氧化作用和抗氧化活性程度对照射持续时间的依赖性。结果表明,低剂量紫外线辐射(5 - 15分钟)可刺激生长8天的绿色小麦芽苗的抗氧化保护作用。将照射时间增加到30 - 60分钟会导致抗氧化系统抑制脂质过氧化作用。用紫外线对种子进行更长时间的照射(1 - 6小时)会导致芽苗中脂质过氧化水平升高。然而,来自经5 - 6小时照射种子的1 - 2日龄芽苗,由于补偿机制而适应了这种影响。到预照射种子发芽的第8天,抗氧化剂和丙二醛的含量恢复到正常水平。研究了低剂量紫外线照射30分钟的种子中过氧化物酶活性的动态变化。发现种子发芽第三天,过氧化物酶活性先降低,随后略有增加。在胚乳中,该酶的最大活性在发芽第5 - 6天观察到,在根和绿色芽苗中,在发芽第3 - 5天观察到。得出的结论是,抗氧化剂和过氧化物酶参与了抑制种子紫外线照射后形成的自由基的补偿机制。

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