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[小麦种子萌发过程中的抗氧化系统]

[The antioxidant system of wheat seeds during germination].

作者信息

Rogozhin V V, Verkhoturov V V, Kuriliuk T T

机构信息

Yakutsk State Agricultural Academy, ul. Krasil'nikova 15, Yakutsk, 677002 Russia.

出版信息

Izv Akad Nauk Ser Biol. 2001 Mar-Apr(2):165-73.

Abstract

The dynamics of peroxidase activity and antioxidant contents in wheat seeds were studied in the course of 24-hour swelling at 5 degrees C (group 1) and 23 degrees C (group 2). Both parameters were 1.5 times higher in seeds of the first group. In the same seeds, peroxidase activity in the endosperm and seed coat increased by factors of 1.5 and 1.8, respectively. Catalytic constants of wheat seed peroxidase were determined in the reactions of o-dianisidine and ascorbic acid peroxidation. In the pH range studied (pH 5-7), Km proved to change only slightly. In seedlings, an increase in the lipid peroxidation rate was accompanied by an increase in the content of antioxidants. Peroxidase activity increased as the content of antioxidants decreased, and vice versa. Thus, the reciprocal influence of peroxidase and low-molecular antioxidants during seed germination in wheat was revealed.

摘要

研究了小麦种子在5℃(第1组)和23℃(第2组)下24小时膨胀过程中过氧化物酶活性和抗氧化剂含量的动态变化。第一组种子的这两个参数均高出1.5倍。在同一批种子中,胚乳和种皮中的过氧化物酶活性分别提高了1.5倍和1.8倍。在邻联茴香胺和抗坏血酸过氧化反应中测定了小麦种子过氧化物酶的催化常数。在所研究的pH范围内(pH 5 - 7),Km值变化很小。在幼苗中,脂质过氧化速率的增加伴随着抗氧化剂含量的增加。过氧化物酶活性随抗氧化剂含量的降低而增加,反之亦然。因此,揭示了小麦种子萌发过程中过氧化物酶与低分子抗氧化剂之间的相互影响。

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