Takano M, Sakanaka F, Yayama K, Okamoto H
Department of Pharmacology, Faculty of Pharmaceutical Sciences and High Technology Research Center, Kobe Gakuin University, Japan.
Biol Pharm Bull. 2000 Oct;23(10):1239-42. doi: 10.1248/bpb.23.1239.
To characterize the local kallikrein-kinin system, tissue distribution of mRNAs for kininogens, precursor proteins of kinins, such as high-molecular-weight (H-), low-molecular-weight (L-) and T-kininogens, were studied in the rat by means of reverse-transcription polymerase chain reaction (RT-PCR) using a fluorophore Cy5-labeled 5'-primer. High levels of these three kininogen mRNAs were present in the liver. Relatively high levels of H-kininogen mRNA were also detected in the skin, lung, kidney, and testis in a descending order, whereas L-kininogen mRNA was detectable in the lung and brain, but not in the kidney, skin, testis, heart, adrenal gland, or skeletal muscle. T-Kininogen mRNA was present in these tissues, except for skeletal muscle. These findings suggest that the expression of each kininogen mRNA is regulated by the tissue-dependent mechanisms which is closely associated with functions of the kallikrein-kinin system in the tissue.
为了表征局部激肽释放酶-激肽系统，通过使用荧光团Cy5标记的5'-引物的逆转录聚合酶链反应（RT-PCR），研究了大鼠中激肽原（激肽的前体蛋白，如高分子量（H-）、低分子量（L-）和T-激肽原）mRNA的组织分布。这三种激肽原mRNA在肝脏中含量很高。在皮肤、肺、肾和睾丸中也检测到相对较高水平的H-激肽原mRNA，且呈递减顺序，而L-激肽原mRNA在肺和脑中可检测到，但在肾、皮肤、睾丸、心脏、肾上腺或骨骼肌中未检测到。除骨骼肌外，这些组织中均存在T-激肽原mRNA。这些发现表明，每种激肽原mRNA的表达受组织依赖性机制调控，该机制与组织中激肽释放酶-激肽系统的功能密切相关。
