Goubau S, Morck D W, Buret A
Department of Biological Sciences, University of Calgary, Alberta.
Can J Vet Res. 2000 Oct;64(4):238-42.
The expression of the interleukin-8 (IL-8) gene was examined by in situ hybridization in lung tissues from calves experimentally infected with Mannheimia (Pasteurella) haemolytica and treated with tilmicosin. Interleukin-8 mRNA expression was detected in alveolar areas, particularly along interlobular septa, in the lumen, and in the epithelial cells of some bronchioles. In lesional lung tissues from animals that had received tilmicosin, we found large areas with limited inflammation. There was no staining for IL-8 mRNA in these areas. In contrast, in strongly inflamed areas, the same patterns and intensities of staining for IL-8 mRNA were detected in tilmicosin- and sham-treated animals. We conclude that tilmicosin does not affect the expression of IL-8 mRNA in tissue showing microscopic signs of inflammation. Together with previous reports, this supports the view that the pro-apoptotic properties of tilmicosin on neutrophils do not compromise the host defense mechanisms required to control the infection.
通过原位杂交技术检测了实验性感染溶血曼氏杆菌（溶血巴斯德氏菌）并用替米考星治疗的犊牛肺组织中白细胞介素-8（IL-8）基因的表达。在肺泡区域检测到白细胞介素-8信使核糖核酸（mRNA）表达，尤其是沿小叶间隔、管腔以及一些细支气管的上皮细胞处。在接受替米考星治疗的动物的病变肺组织中，我们发现大片炎症受限区域。这些区域未检测到IL-8 mRNA染色。相比之下，在炎症强烈的区域，替米考星治疗组和假处理组动物中检测到的IL-8 mRNA染色模式和强度相同。我们得出结论，替米考星不影响显示微观炎症迹象的组织中IL-8 mRNA的表达。与先前的报道一起，这支持了替米考星对中性粒细胞的促凋亡特性不会损害控制感染所需的宿主防御机制这一观点。
